白皮杉醇对牛血清白蛋白结构和活性的影响：多光谱和分子建模研究。
Effects of piceatannol on the structure and activities of bovine serum albumin: A multi-spectral and molecular modeling studies.
机构信息
Center for Molecular Metabolism, Nanjing University of Science and Technology, B508, No. 364 Building, 200 Xiaolingwei Street, Nanjing, 210094, People's Republic of China.
Center for Molecular Metabolism, Nanjing University of Science and Technology, B508, No. 364 Building, 200 Xiaolingwei Street, Nanjing, 210094, People's Republic of China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Mar 5;228:117706. doi: 10.1016/j.saa.2019.117706. Epub 2019 Oct 31.
Piceatannol (PIC) displays a wide spectrum of biological activities, such as antioxidation, antibacterial activity and anti-inflammation, but the biochemical and molecular mechanism is not fully understood. In this study, the interaction of PIC with bovine serum albumin (BSA) was studied by fluorescence spectroscopy, ultraviolet-visible absorption spectroscopy, circular dichroism spectroscopy and molecular simulation. The effects of PIC on BSA non-enzymatic glycosylation, fibrillation, thermal stability, and structure information were also studied. The results showed that the formation of PIC-BSA complex by mainly hydrogen-bonding forces resulted in the conformational changes of protein. PIC inhibited the formation of β-sheets structures of BSA. BSA still maintained the esterase-like good activity in the presence of PIC. In addition, PIC significantly reduced the degree of BSA glycosylation. These results provided a basis for the molecular interaction between PIC and protein, and suggested the potential effect of PIC in preventing the progression of diabetes mellitus.
白皮杉醇（PIC）具有广泛的生物活性，如抗氧化、抗菌活性和抗炎作用，但生化和分子机制尚不完全清楚。本研究采用荧光光谱法、紫外可见吸收光谱法、圆二色光谱法和分子模拟法研究了 PIC 与牛血清白蛋白（BSA）的相互作用。还研究了 PIC 对 BSA 非酶糖化、聚集、热稳定性和结构信息的影响。结果表明，PIC 通过氢键主要形成 PIC-BSA 复合物，导致蛋白质构象发生变化。PIC 抑制了 BSA 的β-折叠结构的形成。在 PIC 的存在下，BSA 仍然保持良好的酯酶样活性。此外，PIC 显著降低了 BSA 的糖化程度。这些结果为 PIC 与蛋白质之间的分子相互作用提供了依据，并提示 PIC 在预防糖尿病进展方面的潜在作用。
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