Department of Metabolic Biochemistry, Pitié-Salpêtrière-Charles Foix Hospital, AP-HP, Paris, France; INSERM U 1022 CNRS UMR 8258, Chimie ParisTech, PSL Research University, Laboratory of Chemical and Biological Technologies for Health (UTCBS), Faculty of Pharmacy of Paris, University of Paris, France.
National Institute for Health and Medical Research (INSERM), UMR ICAN 1166, Sorbonne University Paris 6, Pitié-Salpêtrière - Charles Foix Hospital, AP-HP, Paris, France.
Nutr Metab Cardiovasc Dis. 2020 Jan 3;30(1):33-39. doi: 10.1016/j.numecd.2019.07.022. Epub 2019 Aug 3.
High-density lipoprotein (HDL) particles play atheroprotective roles by their ability to efflux cholesterol from foam cells and to protect low-density lipoproteins (LDLs) from oxidative damage in the arterial intima. We hypothesized that antioxidative properties of HDLs can be attenuated in the oxygen-rich prooxidative arterial environment, contributing to the development of atherosclerosis. To evaluate this hypothesis, we compared antioxidative activity of HDLs from arterial and venous plasmas.
Arterial and venous blood samples were simultaneously obtained from 16 patients (age 68 ± 10 years; 75% males) presenting with ischemic or valvular heart disease. Major HDL subfractions and total HDLs were isolated by density gradient ultracentrifugation and their chemical composition and the capacity to protect LDLs from in vitro oxidation were evaluated. HDL-cholesterol, triglycerides and apolipoprotein (apo) B-100 levels were slightly but significantly reduced by -4 to -8% (p < 0.01) in the arterial vs. venous samples. Total mass of HDL subpopulations was similar and HDL subpopulations did not reveal marked compositional differences between the arterial and venous circulation. Potent antioxidative activity of the small, dense HDL3c subpopulation was significantly reduced in the particles of arterial origin vs. their counterparts from venous plasma (increase of +21% in the propagation rate of LDL oxidation, p < 0.05). Interestingly, antioxidative properties of venous HDLs were enhanced in statin-treated patients relative to untreated subjects.
Antioxidative properties of small, dense HDLs from arterial plasma are attenuated as compared to the particles of venous origin, consistent with the development of atherosclerosis in the arterial wall.
高密度脂蛋白（HDL）颗粒通过从泡沫细胞中流出胆固醇和保护低密度脂蛋白（LDL）免受动脉内膜中氧化损伤的能力发挥抗动脉粥样硬化作用。我们假设富含氧气的促氧化动脉环境会降低 HDL 的抗氧化特性，从而导致动脉粥样硬化的发展。为了评估这一假设，我们比较了来自动脉和静脉血浆的 HDL 的抗氧化活性。
同时从 16 名患有缺血性或瓣膜性心脏病的患者（年龄 68±10 岁；75%为男性）中采集动脉和静脉血样。通过密度梯度超速离心分离主要的 HDL 亚组分和总 HDL，并评估其化学组成和保护 LDL 体外氧化的能力。与静脉样本相比，动脉样本中 HDL-胆固醇、甘油三酯和载脂蛋白（apo）B-100 水平分别降低了-4%至-8%（p<0.01）。HDL 亚群的总质量相似，HDL 亚群在动脉和静脉循环之间没有明显的组成差异。小而密的 HDL3c 亚群的强抗氧化活性在动脉来源的颗粒中明显低于静脉血浆中的相应颗粒（LDL 氧化的传播速率增加+21%，p<0.05）。有趣的是，与未治疗的患者相比，他汀类药物治疗的患者静脉 HDL 的抗氧化特性增强。
与静脉来源的颗粒相比，动脉血浆中小而密的 HDL 的抗氧化特性减弱，这与动脉壁中动脉粥样硬化的发展一致。
