Department of Cardiology, West China Hospital of Sichuan University, Chengdu, China.
Clin Exp Hypertens. 2010 Jan;32(1):13-20. doi: 10.3109/10641960902929487.
The objective of this study was to evaluate high-density lipoprotein (HDL) antioxidative activity and its possible influencing factors in patients with essential hypertension and to investigate the correlations between HDL antioxidative activity and the carotid arterial intima-media thickness (CIMT). Thirty-three patients with essential hypertension and 32 healthy people as control were included. High- and low-density lipoprotein in plasma were isolated by one-step density gradient ultracentrifugation, and induced oxidation with external Cu(2+). Antioxidant activity of HDL, lag time, and lipid peroxidation degree were determined by spectrophotometric and thiobarbituric acid reactive substances (TBARS) methods. Paraoxonase 1 (PON1) activity in serum was measured with continuous monitoring using phenylacetate as a substrate. The CIMT was measured with a high-resolution ultrasound Doppler system. In patients with essential hypertension, the inhibitory effect of HDL on low-density lipoprotein (LDL) oxidation and the PON1 activity were reduced (72.29 +/- 2.03)% vs. (80.91 +/- 2.06)%, and (112.21 +/- 8.64)u/ml vs. (146.43 +/- 8.79)u/ml (all P < 0.05). The lag time of oxidation and the lipid peroxidation between the hypertensive group and the control group did not show a statistically significant difference. Multiple stepwise regression analysis revealed that HDL antioxidative activity might be affected by PON1 activity (P = 0.004), diastolic pressure (P = 0.004), sex (P = 0.006), and that CIMT might be affected by HDL antioxidative activity (P = 0.030), systolic pressure (P = 0.026), and total cholesterol level (P = 0.033). The HDL antioxidative activity is reduced in patients with essential hypertension and significantly affected by sex. The CIMT was negatively correlated with HDL antioxidative activity, which suggests that decreased HDL antioxidative activity may be one of the important determinants for the development of atherosclerosis in patients with essential hypertension.
本研究旨在评估原发性高血压患者高密度脂蛋白（HDL）的抗氧化活性及其可能的影响因素，并探讨 HDL 抗氧化活性与颈动脉内膜中层厚度（CIMT）的相关性。共纳入 33 例原发性高血压患者和 32 名健康对照者。采用一步密度梯度超速离心法分离血浆中的高、低密度脂蛋白，并用外源性 Cu(2+)诱导其氧化。采用分光光度法和硫代巴比妥酸反应物质（TBARS）法测定 HDL 的抗氧化活性、延迟时间和脂质过氧化程度。采用苯乙酸连续监测法测定血清对氧磷酶 1（PON1）活性。采用高分辨率超声多普勒系统测量 CIMT。结果显示，与对照组相比，原发性高血压患者 HDL 对低密度脂蛋白（LDL）氧化的抑制作用和 PON1 活性降低（72.29 +/- 2.03）%比（80.91 +/- 2.06）%，（112.21 +/- 8.64）u/ml 比（146.43 +/- 8.79）u/ml（均 P < 0.05）。两组间 LDL 氧化的延迟时间和脂质过氧化无统计学差异。多元逐步回归分析显示，HDL 抗氧化活性可能受 PON1 活性（P = 0.004）、舒张压（P = 0.004）、性别（P = 0.006）的影响，CIMT 可能受 HDL 抗氧化活性（P = 0.030）、收缩压（P = 0.026）和总胆固醇水平（P = 0.033）的影响。原发性高血压患者 HDL 抗氧化活性降低，且明显受性别影响。CIMT 与 HDL 抗氧化活性呈负相关，提示 HDL 抗氧化活性降低可能是原发性高血压患者动脉粥样硬化发展的重要决定因素之一。
