Korir Robert C, Everts Kathryne L, Micallef Shirley A
Department of Plant Science and Landscape Architecture, University of Maryland, College Park, Maryland.
Lower Eastern Shore Research and Education Center, University of Maryland, Salisbury, Maryland.
Foodborne Pathog Dis. 2020 Jun;17(6):388-395. doi: 10.1089/fpd.2019.2721. Epub 2019 Nov 22.
Melons are perishable fruit of high food safety risk, grown in contact with soil and soil-borne organisms. To assess whether food safety risk could be augmented by the presence of soil-borne fungi, this study investigated the relationship between spp. that were isolated from the surface of melon and the foodborne pathogen . In four repeated trials, rind discs from cultivars, Arava, Athena, Dulce Nectar, Jaune de Canaries, and Sivan fruit, grown in the field and in high tunnels in Maryland were inoculated separately with isolates, . , . , . , and , with no inoculation serving as a control and incubated at 25°C. Newport was inoculated onto melon discs 4 d post inoculation and recovered 24 h later. Melon cultivar impacted the retrieval of Newport. In all four replicated experiments, one or more of the netted varieties, Arava, Athena, and Sivan, yielded higher Newport counts than one or both smooth-rind melons, Jaune de Canaries and Dulce Nectar ( < 0.05). inoculation did not have a marked impact on retrieval. The average count recovered was 5.0 log colony-forming unit (CFU)/mL for both -inoculated and uninoculated melons. However, in one trial, Newport counts recovered from inoculated melons were higher than all other treatments (8.6 log CFU/mL; < 0.001), due to high levels of recovered from Jaune de Canaries compared with other experiments. The food safety risk of melon did not appear to be enhanced by postharvest colonization with saprophytic spp. However, melons with netted rinds appeared to favor colonization compared with smooth melons. Choice of melon cultivar may be an important consideration in reducing colonization risk in areas where may be endemic in the environment.
甜瓜是具有高食品安全风险的易腐水果，生长过程中会接触土壤和土壤传播的生物。为评估土壤传播真菌的存在是否会增加食品安全风险，本研究调查了从甜瓜表面分离出的 spp. 与食源性病原体 之间的关系。在四项重复试验中，将在马里兰州田间和高棚中种植的阿拉瓦、雅典娜、甜蜜花蜜、加那利黄、西万等品种的甜瓜果盘，分别接种 分离株，即 、 、 、 、 和 ，不接种作为对照，并在 25°C 下培养。接种 4 天后，将纽波特菌接种到甜瓜果盘上，24 小时后回收。甜瓜品种影响纽波特菌的回收。在所有四项重复实验中，一个或多个网纹品种，如阿拉瓦、雅典娜和西万，其纽波特菌计数高于一个或两个光皮甜瓜品种，加那利黄和甜蜜花蜜（ ＜0.05）。接种 对纽波特菌的回收没有显著影响。接种和未接种 的甜瓜回收的平均 计数均为 5.0 对数菌落形成单位（CFU）/mL。然而，在一项试验中，接种 的甜瓜回收的纽波特菌计数高于所有其他处理（8.6 对数 CFU/mL； ＜0.001），这是因为与其他实验相比，从加那利黄中回收的 水平较高。收获后被腐生 spp. 定殖似乎并未增加甜瓜的食品安全风险。然而，与光皮甜瓜相比，网纹皮甜瓜似乎更有利于 定殖。在 在环境中可能流行的地区，选择甜瓜品种可能是降低 定殖风险的一个重要考虑因素。
