School of Animal & Comparative Biomedical Sciences, University of Arizona, Tucson, AZ, 85721, USA; National Center of Excellence for Melon at the Vegetable and Fruit Improvement Center of Texas A&M University, College Station, TX, 77845, USA.
School of Animal & Comparative Biomedical Sciences, University of Arizona, Tucson, AZ, 85721, USA; Current address: College of Life Science, Ningde Normal University, Ningde, Fujian, China; National Center of Excellence for Melon at the Vegetable and Fruit Improvement Center of Texas A&M University, College Station, TX, 77845, USA.
Food Microbiol. 2022 Feb;101:103876. doi: 10.1016/j.fm.2021.103876. Epub 2021 Jul 29.
The efficacy of plant-based antimicrobials against Salmonella Newport and Listeria monocytogenes on melon rinds was evaluated. Four cantaloupe and 3 honeydew melon varieties grown in Georgia, Arizona, Texas, North Carolina, Indiana and California were tested. Melon rinds (10 g pieces) were inoculated with 5-6 log CFU/10 g rind of S. Newport or L. monocytogenes. Samples were then immersed in 5 % olive extract or 0.5 % oregano oil antimicrobial solution and gently agitated for 2 min. Samples were stored at 4 °C and surviving populations of both bacteria were enumerated at days 0 and 3. Plant-based antimicrobials reduced S. Newport and L.monocytogenes population on all rind samples, regardless of the melon types, varieties or growing locations. Compared to the control, antimicrobial treatments caused up to 3.6 and 4.0 log reductions in populations of Salmonella and L. monocytogenes, respectively. In most cases, plant-based antimicrobial treatments reduced pathogen populations to below the detection limit (1 log CFU/g) at day 3. In general, oregano oil had better antimicrobial activity than olive extract and the antimicrobial treatments were more effective on Salmonella than on L. monocytogenes. The plant-based antimicrobial treatments exhibited better microbial reductions on honeydews than on cantaloupes. These antimicrobials could potentially be used as sanitizers for decontaminating melons.
研究了植物源抗菌剂对甜瓜表皮中肠炎沙门氏菌和单增李斯特菌的抑菌效果。试验选用了在佐治亚州、亚利桑那州、德克萨斯州、北卡罗来纳州、印第安纳州和加利福尼亚州种植的 4 种哈密瓜和 3 种蜜瓜。将甜瓜皮（10g 块）用 5-6 对数 CFU/10g 皮肠炎沙门氏菌或单增李斯特菌接种。然后将样品浸入 5%橄榄油提取物或 0.5%牛至油抗菌溶液中，轻轻搅拌 2 分钟。将样品储存在 4°C 下，在第 0 天和第 3 天对两种细菌的存活种群进行计数。植物源抗菌剂可降低所有果皮样品中肠炎沙门氏菌和单增李斯特菌的种群，而与甜瓜类型、品种或种植地点无关。与对照相比，抗菌处理分别导致肠炎沙门氏菌和单增李斯特菌的种群减少了 3.6 和 4.0 个对数。在大多数情况下，植物源抗菌处理可将病原菌种群减少到 3 天检测限以下（1 对数 CFU/g）。总的来说，牛至油的抗菌活性优于橄榄油提取物，且抗菌处理对肠炎沙门氏菌的效果优于单增李斯特菌。这些植物源抗菌剂对蜜瓜的微生物减少效果优于哈密瓜。这些抗菌剂可能有潜力作为消毒剂用于瓜类的消毒。
