Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences , Wuhan University , Wuhan 430072 , China.
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry , Jilin University , Changchun 130012 , China.
Anal Chem. 2020 Jan 7;92(1):535-542. doi: 10.1021/acs.analchem.9b04156. Epub 2019 Dec 10.
The fluorescence imaging in the second near-infrared window (NIR-II, 1000-1700 nm) has emerged as a new method for imaging and attracted considerable attention in the past decade. Owing to the suppressed photon scattering and diminished autofluorescence, fluorescence imaging in NIR-II window can afford deep tissue penetration depth with high clarity. Inorganic nanoparticle-based fluorescent probes in the NIR-II window have greatly prospered the field into a development stage because of their superior traits, including adjustable emission covering the whole NIR-II window and abundant surface functional groups that facilitate chemical modification and bioconjugation, In this Feature, we introduce the unique imaging performance of the NIR-II optical window and highlight the latest development of noninvasive biological fluorescent imaging in NIR-II window using inorganic nanoparticle-based probes. A perspective on the challenge and future direction of inorganic nanoparticle-based NIR-II probes is also discussed.
近红外二区（NIR-II，1000-1700nm）的荧光成像是一种新兴的成像方法，在过去十年中引起了相当大的关注。由于抑制了光子散射和自发荧光，NIR-II 窗口中的荧光成像是能够提供高清晰度的深层组织穿透深度。基于无机纳米粒子的荧光探针在 NIR-II 窗口中得到了极大的发展，因为它们具有可调的发射覆盖整个 NIR-II 窗口和丰富的表面官能团，这有利于化学修饰和生物偶联，在这篇特写中，我们介绍了 NIR-II 光学窗口的独特成像性能，并强调了使用基于无机纳米粒子的探针进行非侵入性生物荧光成像的最新进展。还讨论了基于无机纳米粒子的 NIR-II 探针的挑战和未来方向。
