Instituto de Salud y Ambiente del Litoral (ISAL; UNL-CONICET), Facultad de Bioquímica y Ciencias Biológicas (FBCB), Universidad Nacional del Litoral (UNL), Santa Fe, Argentina; Cátedra de Fisiología Humana, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
Instituto de Salud y Ambiente del Litoral (ISAL; UNL-CONICET), Facultad de Bioquímica y Ciencias Biológicas (FBCB), Universidad Nacional del Litoral (UNL), Santa Fe, Argentina.
Mol Cell Endocrinol. 2020 Feb 5;501:110658. doi: 10.1016/j.mce.2019.110658. Epub 2019 Nov 19.
Glyphosate-based herbicide (GBH) exposure is known to have adverse effects on endocrine-related tissues. Here, we aimed to determine whether early postnatal exposure to a GBH induces long-term effects on the rat mammary gland. Thus, female Wistar pups were injected with saline solution (Control) or GBH (2 mg glyphosate/kg/day) on postnatal days (PND) 1, 3, 5 and 7. At 20 months of age, mammary gland samples were collected to determine histomorphological features, proliferation index and the expression of steroid hormone receptors expression, by immunohistochemistry, and serum samples were collected to assess 17β-estradiol (E2) and progesterone (P4) levels. GBH exposure induced morphological changes evidenced by a higher percentage of hyperplastic ducts and a fibroblastic-like stroma in the mammary gland. GBH-treated rats also showed a high expression of steroid hormone receptors in hyperplastic ducts. The results indicate that early postnatal exposure to GBH induces long-term alterations in the mammary gland morphology of aging female rats.
草甘膦基除草剂（GBH）暴露已知对与内分泌相关的组织有不良影响。在这里，我们旨在确定新生后暴露于 GBH 是否会对大鼠乳腺产生长期影响。因此，雌性 Wistar 幼崽在出生后第 1、3、5 和 7 天分别注射生理盐水（对照）或 GBH（2mg 草甘膦/公斤/天）。在 20 个月大时，收集乳腺样本，通过免疫组织化学检测组织形态学特征、增殖指数和类固醇激素受体表达，收集血清样本评估 17β-雌二醇（E2）和孕酮（P4）水平。GBH 暴露诱导了形态学变化，表现为乳腺中增生导管的百分比更高和纤维母细胞样基质。GBH 处理的大鼠在增生导管中也表现出高水平的类固醇激素受体。结果表明，新生后早期暴露于 GBH 会导致老年雌性大鼠乳腺形态长期改变。
