Heilongjiang University of Chinese Medicine, Harbin, 150040, P. R. China.
Crop Research Institute, Heilongjiang University, Harbin, 150080, P. R. China.
Rapid Commun Mass Spectrom. 2020 Mar 15;34(5):e8600. doi: 10.1002/rcm.8600.
In view of the unique properties of wooden materials as electrospray emitters, a novel wooden capillary electrospray ionization (WC-ESI) device was fabricated. The performance of a wooden capillary as an electrospray emitter was investigated by using a wooden capillary instead of the metal emitter of commercial ESI sources.
The mass spectrometric measurement of baicalein, emodin and myoglobin was carried out by using wooden capillary (WC) and metal capillary (MC) ESI sources. Contrasting analysis of signal intensity between WC and MC electrospray ionization mass spectrometry (ESI-MS) was implemented at different sample flow rates. The effect of WC-ESI-MS and MC-ESI-MS was evaluated experimentally with electrospray solutions in different solvent ratios.
Generally, the signal generated by WC-ESI-MS was much stronger than that obtained by MC-ESI-MS. In particular, the MS signal in negative ion mode was very strong, which may solve the long-standing problem of low MS signals in negative ion mode, and fully improve the detection efficiency of ESI-MS.
The signal intensity produced by WC-ESI-MS is significantly higher than that from MC-ESI-MS, and polymerization and electrolysis are reduced; therefore, the spectra become simpler. In addition, it is also tolerant to high flow rates and high aqueous phase samples.
鉴于木质材料作为电喷雾发射器的独特性质，我们制造了一种新型的木质毛细管电喷雾离子化（WC-ESI）装置。通过使用木质毛细管代替商业 ESI 源的金属发射器，研究了木质毛细管作为电喷雾发射器的性能。
采用木质毛细管（WC）和金属毛细管（MC）ESI 源对黄芩素、大黄素和肌红蛋白进行了质谱测量。在不同的样品流速下，对 WC 和 MC 电喷雾电离质谱（ESI-MS）之间的信号强度进行了对比分析。通过在不同溶剂比的电喷雾溶液中进行实验，评估了 WC-ESI-MS 和 MC-ESI-MS 的效果。
通常情况下，WC-ESI-MS 产生的信号比 MC-ESI-MS 强得多。特别是在负离子模式下，MS 信号非常强，这可能解决了负离子模式下 MS 信号弱的长期问题，并且充分提高了 ESI-MS 的检测效率。
WC-ESI-MS 产生的信号强度明显高于 MC-ESI-MS，并且减少了聚合和电解；因此，谱图变得更简单。此外，它还能耐受高流速和高水相样品。
