Institute of Medical Virology, University of Zurich, Zurich, Switzerland.
Division of Infectious Diseases and Hospital Epidemiology, University Children's Hospital Zurich, Zurich, Switzerland.
Diagn Microbiol Infect Dis. 2020 Feb;96(2):114924. doi: 10.1016/j.diagmicrobio.2019.114924. Epub 2019 Nov 7.
Sapovirus (SaV) and astrovirus (AstV) increasingly are recognized as cause of acute viral gastroenteritis (AGE). We evaluated the real-time RT-PCR assays RIDA®GENE SaV and viral stool panel II (RGN RT-PCR) for detection of SaV, AstV, adenovirus (AdV) F40/41 and rotavirus (RoV) in clinical stool samples (n = 69). Results were compared with reference singleplex RT-PCRs. The sensitivity for SaV, AstV and RoV are 100%, the specificity ranges from 98.1% to 100%. In 10 out of 11 AdV (all types) samples, the RGN RT-PCR for AdV F40/41 displayed negative results. Retrospectively, 196 stool specimens from adult patients previously tested negative for norovirus (NoV) were analyzed. In about 10% of NoV-negative stool samples, AdV (n = 9), RoV (n = 6), AstV (n = 3) or SaV (n = 3) were found. The RGN RT-PCR assays are useful for detection of enteric viruses other than NoV. This study emphasizes the need for further testing of NoV-negative stool samples in patients with AGE.
肠道病毒（SaV）和星状病毒（AstV）越来越被认为是急性病毒性胃肠炎（AGE）的病因。我们评估了实时 RT-PCR 检测试剂盒 RIDA®GENE SaV 和病毒粪便组套 II（RGN RT-PCR）在临床粪便样本（n=69）中检测 SaV、AstV、腺病毒（AdV）F40/41 和轮状病毒（RoV）的能力。结果与参考的单重 RT-PCR 进行了比较。SaV、AstV 和 RoV 的灵敏度为 100%，特异性范围为 98.1%至 100%。在 11 个 AdV（所有类型）样本中的 10 个中，RGN RT-PCR 对 AdV F40/41 显示阴性结果。回顾性分析了先前检测诺如病毒（NoV）为阴性的 196 份成人患者粪便标本。在约 10%的 NoV 阴性粪便样本中，发现了 AdV（n=9）、RoV（n=6）、AstV（n=3）或 SaV（n=3）。RGN RT-PCR 检测试剂盒对于检测除 NoV 以外的肠道病毒非常有用。本研究强调了对 AGE 患者的 NoV 阴性粪便样本进行进一步检测的必要性。
