Bennett Susan, Gunson Rory N
West of Scotland Specialist Virology Centre, United Kingdom.
West of Scotland Specialist Virology Centre, United Kingdom.
J Virol Methods. 2017 Apr;242:30-34. doi: 10.1016/j.jviromet.2016.12.016. Epub 2016 Dec 28.
Viral gastroenteritis is a major health problem with significant morbidity and economic consequences. Viral gastroenteritis is caused by a number of viruses, including norovirus, rotavirus, adenovirus, astrovirus, and sapovirus. Conventional diagnosis is based on direct antigen detection and electron microscopy, however enzyme immunoassay's are insensitive and not available for all relevant pathogens, and electron microscope (EM) is no longer routinely carried out in most laboratories. Most laboratories now offer norovirus real-time PCR testing however the availability of other assays is variable. Commercial methods for the detection of inflectional intestinal disease (IID) are available but these can be expensive and are not commonly used. This paper describes the development of a single multiplex assay for the simultaneous detection of adenovirus, astrovirus, rotavirus and sapovirus from stool samples. The multiplex was evaluated by assessing endpoint sensitivity, specificity, panel of clinical samples, quality control (QC) panel and the robustness and reproducibility of the multiplex.
病毒性肠胃炎是一个严重的健康问题，会导致显著的发病率和经济后果。病毒性肠胃炎由多种病毒引起，包括诺如病毒、轮状病毒、腺病毒、星状病毒和札幌病毒。传统诊断基于直接抗原检测和电子显微镜检查，然而酶免疫测定法不敏感，且并非适用于所有相关病原体，并且大多数实验室不再常规进行电子显微镜（EM）检查。现在大多数实验室提供诺如病毒实时聚合酶链反应检测，但其他检测方法的可用性各不相同。有用于检测感染性肠道疾病（IID）的商业方法，但这些方法可能成本高昂且不常用。本文描述了一种用于同时从粪便样本中检测腺病毒、星状病毒、轮状病毒和札幌病毒的单一多重检测方法的开发。通过评估终点灵敏度、特异性、临床样本组、质量控制（QC）组以及该多重检测方法的稳健性和可重复性对该多重检测方法进行了评估。
