姜黄素治疗实验性外阴阴道念珠菌病的疗效
Efficacy of curcumin in the treatment of experimental vulvovaginal candidiasis.
作者信息
Andrade Jessica Tauany, Fantini de Figueiredo Geisa, Cruz Luisa Ferreira, Eliza de Morais Sarah, Souza Carla Daiane Ferreira, Pinto Flávia Carmo Horta, Ferreira Jaqueline Maria Siqueira, Araújo Marcelo Gonzaga de Freitas
机构信息
Laboratory of Medical Microbiology, Federal University of São João Del-Rei, Campus Centro Oeste Dona Lindu, Divinópolis, Minas Gerais, Brazil.
Laboratory of Pharmacology, Federal University of São João Del-Rei, Campus Centro Oeste Dona Lindu, Divinópolis, Minas Gerais, Brazil.
出版信息
Rev Iberoam Micol. 2019 Oct-Dec;36(4):192-199. doi: 10.1016/j.riam.2019.01.003. Epub 2019 Nov 19.
BACKGROUND
Candida albicans is the main agent that causes vulvovaginal candidiasis. Resistance among isolates to azole antifungal agents has been reported.
AIMS
Due to the well-known antifungal potential of curcumin, the purpose of this work was to evaluate the in vitro anticandidal activity of curcumin and its effect in the treatment of experimental vulvovaginal candidiasis.
METHODS
The anticandidal activity of curcumin was investigated against eight Candida strains by the broth microdilution assay, and its mechanism of action was evaluated by testing the binding to ergosterol. Then, the effect of curcumin in the treatment of vulvovaginal candidiasis was evaluated in an immunosuppressed, estrogen treated rat model.
RESULTS
Curcumin showed minimum inhibitory concentration values of 125-1000μg/ml, and the best result was observed against Candida glabrata. The compound was shown to be able to bind to the ergosterol present in the membrane, event that may be the mechanism of action. In addition, in the in vivo model of vulvovaginal candidiasis with C. albicans, treatments reduced the vaginal fungal burden in infected rats after seven days of treatment with different doses.
CONCLUSIONS
Curcumin could be considered a promising effective antifungal agent in the treatment of vulvovaginal candidiasis.
背景
白色念珠菌是引起外阴阴道念珠菌病的主要病原体。已有报道分离株对唑类抗真菌药物产生耐药性。
目的
鉴于姜黄素具有众所周知的抗真菌潜力，本研究旨在评估姜黄素的体外抗念珠菌活性及其在治疗实验性外阴阴道念珠菌病中的作用。
方法
采用肉汤微量稀释法研究姜黄素对8株念珠菌的抗念珠菌活性，并通过检测其与麦角固醇的结合来评估其作用机制。然后，在免疫抑制、雌激素处理的大鼠模型中评估姜黄素治疗外阴阴道念珠菌病的效果。
结果
姜黄素的最低抑菌浓度值为125 - 1000μg/ml，对光滑念珠菌的效果最佳。该化合物被证明能够与细胞膜中的麦角固醇结合，这可能是其作用机制。此外，在白色念珠菌引起的外阴阴道念珠菌病体内模型中，不同剂量治疗7天后，治疗降低了感染大鼠阴道内的真菌负荷。
结论
姜黄素可被认为是治疗外阴阴道念珠菌病的一种有前景的有效抗真菌药物。
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