Mosca Valeria, Arita Glaucia Sayuri, Sakita Karina Mayumi, Rodrigues-Vendramini Franciele Abigail Vilugron, Faria Daniella Renata, Conrado Pollyanna Cristina Vincenzi, Galinari Camila Barros, Kioshima Érika Seki, Becker Tania Cristina Alexandrino, de Souza Bonfim-Mendonça Patrícia, Svidzinski Terezinha Inez Estivalet
Department of Clinical Analysis and Biomedicine, Laboratory of Medical Mycology, State University of Maringá, Maringá, Paraná, Brazil.
Department of Basic Health Sciences, Laboratory of Pathology, State University of Maringá, Maringá, Paraná, Brazil.
Braz J Microbiol. 2025 Mar;56(1):415-423. doi: 10.1007/s42770-024-01602-9. Epub 2025 Jan 10.
Vulvovaginal candidiasis (VVC) represents the second cause of vaginal infections in childbearing-age women. It mainly affects the vulva and vagina; however, other organs can be compromised, with consequences that are not well known yet. To evaluate the ability of Candida albicans, inoculated into the vaginal lumen of mice, to migrate to the uterus and ovaries. Female Balb/c mice, 6 weeks old, were estrogenized and infected intravaginally with 1 × 10 CFU/ml of C. albicans for the dual objectives of histopathological evaluation and fungal burden quantification in the vaginal, uterus, and ovary tissues, on days 1, 3, and 5 post-infection. Estrogenized and infected mice presented vaginal exudate and an edematous and erythematous uterus. Vaginal histopathological analysis showed intense desquamation, keratinization, fungal structures, and an inflammatory reaction one day after infection. In the uterus, an inflammatory response and a few fungi from the third day onwards were observed. Similarly, the ovary showed the same changes, but at a lower intensity. A high fungal burden was detected in the vaginal tissue from the first day, reaching nearly 6 log CFU/g on the fifth day. C. albicans inoculated into the vaginal lumen of estrogenized mice was able to invade the vaginal tissue one-day post-infection and was detected in the uterus and ovaries within three days.
外阴阴道念珠菌病（VVC）是育龄期女性阴道感染的第二大原因。它主要影响外阴和阴道；然而，其他器官也可能受到影响，其后果尚不清楚。为了评估接种到小鼠阴道腔内的白色念珠菌迁移至子宫和卵巢的能力。对6周龄的雌性Balb/c小鼠进行雌激素处理，并在感染后第1天、第3天和第5天经阴道接种1×10 CFU/ml白色念珠菌，以对外阴、子宫和卵巢组织进行组织病理学评估和真菌负荷定量分析。经雌激素处理并感染的小鼠出现阴道分泌物以及子宫水肿和红斑。阴道组织病理学分析显示，感染后一天出现强烈的脱屑、角化、真菌结构和炎症反应。在子宫中，从第三天起观察到炎症反应和少量真菌。同样，卵巢也出现了相同的变化，但程度较轻。从第一天起就在阴道组织中检测到高真菌负荷，在第五天达到近6 log CFU/g。接种到经雌激素处理小鼠阴道腔内的白色念珠菌在感染后一天能够侵入阴道组织，并在三天内在子宫和卵巢中被检测到。
