APC Microbiome Ireland, School of Microbiology, University College Cork, Cork, Ireland.
APC Microbiome Ireland, School of Microbiology, University College Cork, Cork, Ireland.
Cell Host Microbe. 2019 Dec 11;26(6):764-778.e5. doi: 10.1016/j.chom.2019.10.009. Epub 2019 Nov 19.
The human gut virome is thought to significantly impact the microbiome and human health. However, most virome analyses have been performed on a limited fraction of known viruses. Using whole-virome analysis on a published keystone inflammatory bowel disease (IBD) cohort and an in-house ulcerative colitis dataset, we shed light on the composition of the human gut virome in IBD beyond this identifiable minority. We observe IBD-specific changes to the virome and increased numbers of temperate phage sequences in individuals with Crohn's disease. Unlike prior database-dependent methods, no changes in viral richness were observed. Among IBD subjects, the changes in virome composition reflected alterations in bacterial composition. Furthermore, incorporating both bacteriome and virome composition offered greater classification power between health and disease. This approach to analyzing whole virome across cohorts highlights significant IBD signals, which may be crucial for developing future biomarkers and therapeutics.
人类肠道病毒组被认为对微生物组和人类健康有重大影响。然而，大多数病毒组分析都是针对有限数量的已知病毒进行的。通过对已发表的炎症性肠病（IBD）队列和内部溃疡性结肠炎数据集进行全病毒组分析，我们揭示了 IBD 中除了可识别的少数病毒之外，人类肠道病毒组的组成情况。我们观察到 IBD 患者的病毒组具有特异性变化，并且在克罗恩病患者中，温和噬菌体序列的数量增加。与之前基于数据库的方法不同，我们没有观察到病毒丰富度的变化。在 IBD 患者中，病毒组组成的变化反映了细菌组成的改变。此外，将细菌组和病毒组组成结合起来，可以在健康和疾病之间提供更好的分类能力。这种在队列中分析全病毒组的方法突出了 IBD 的重要信号，这对于开发未来的生物标志物和治疗方法可能至关重要。
