Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Str., Bl. 25, 1113, Sofia, Bulgaria.
Faculty of Chemistry and Pharmacy, Sofia University "St. Kliment Ohridski", James Bourchier Blvd. N1, 1164, Sofia, Bulgaria.
J Trace Elem Med Biol. 2020 Mar;58:126431. doi: 10.1016/j.jtemb.2019.126431. Epub 2019 Nov 12.
Sodium nitrite (NaNO) is an inorganic salt with numerous applications in a variety of industries, as well as in medicine. Nevertheless, exposure to high levels of NaNO is toxic for animals and humans. Sodium nitrite intoxication is shown to decrease the activity of major antioxidant defence enzymes which is dependent on the maintenance of specific ion equilibrium. The aim of the present study was to investigate the effect of acute NaNO intoxication on the content of the essential metals iron (Fe), calcium (Ca) and zinc (Zn) in mouse spleen.
Mature male ICR mice were divided into four groups and subjected to acute NaNO exposure by a single intraperitoneal injection of 120 mg/kg body weight. Animals in each group were sacrificed at certain time interval after treatment (1 h, 5 h, 1 day and 2 days). Spleens were excised and processed for atomic absorption spectrometry analysis of Fe, Ca and Zn content.
At the first hour after treatment, a decrease in Fe and Ca levels was observed. One day following NaNO administration, Zn concentration reached its lowest value and Ca levels remained lower, compared to the untreated controls. In contrast, Fe concentration increased on the first and second day after treatment.
The results of the present study demonstrate that acute NaNO intoxication provokes changes in the endogenous levels of Fe, Ca and Zn in mouse spleen. These findings suggest disruption of the ionic balance and impact on the activity of antioxidant defence enzymes.
亚硝酸钠（NaNO）是一种无机盐，在许多行业，以及医学领域都有广泛应用。然而，动物和人类接触高浓度的 NaNO 是有毒的。亚硝酸钠中毒会降低主要抗氧化防御酶的活性，这取决于特定离子平衡的维持。本研究的目的是研究急性 NaNO 中毒对小鼠脾脏中必需金属铁（Fe）、钙（Ca）和锌（Zn）含量的影响。
成熟雄性 ICR 小鼠分为四组，通过单次腹腔注射 120mg/kg 体重的 NaNO 进行急性暴露。每组动物在治疗后（1 小时、5 小时、1 天和 2 天）的特定时间间隔处死。切除脾脏并进行原子吸收光谱分析，以测定 Fe、Ca 和 Zn 的含量。
在治疗后的第一个小时，观察到 Fe 和 Ca 水平下降。NaNO 给药后 1 天，Zn 浓度达到最低值，与未处理对照组相比，Ca 水平仍较低。相反，Fe 浓度在治疗后的第 1 天和第 2 天增加。
本研究的结果表明，急性 NaNO 中毒会引起小鼠脾脏内 Fe、Ca 和 Zn 的内源性水平发生变化。这些发现表明离子平衡的破坏和对抗氧化防御酶活性的影响。
