细胞荧光和免疫组织化学对比检测非霍奇金淋巴瘤骨髓浸润：354 例患者研究。

Cytofluorimetric and immunohistochemical comparison for detecting bone marrow infiltration in non-Hodgkin lymphomas: a study of 354 patients.

机构信息

Laboratory of Clinical Research and Advanced Diagnostics, Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Rionero in Vulture, Pz, Italy.

出版信息

Leuk Res. 2020 Jan;88:106267. doi: 10.1016/j.leukres.2019.106267. Epub 2019 Nov 12.

DOI:10.1016/j.leukres.2019.106267

PMID:31759282

Abstract

Morphological and immunohistochemical (IHC) analysis of bone marrow biopsies (BMB) is routinely performed during staging of patients with non-Hodgkin's lymphoma (NHL). Aiming to evaluate the possible diagnostic value of flow cytometry (FC) on bone marrow aspirates (BMA), as compared with BMB, we retrospectively reviewed BMA specimen of 354 NHL. In 305 cases (86.1 %), there was a concordance between the two investigations. A discordance was detected in 49 cases (14 %): in 33 of these (9.3 % of total population), FC analysis of BMA was positive, whereas BMB, supported by IHC, was negative; in 16 (4.5 % of total population), FC did not detected lymphoid infiltration, while BMB was positive. Although the clinical implications of such an observation remain unclear, we think our results may be useful in the context of current staging procedures, also opening a possible future perspective in the setting of minimal measurable disease in these patients.

摘要

骨髓活检（BMB）的形态学和免疫组织化学（IHC）分析通常在非霍奇金淋巴瘤（NHL）患者的分期中进行。为了评估流式细胞术（FC）在骨髓抽吸物（BMA）中的可能诊断价值，并与 BMB 进行比较，我们回顾性分析了 354 例 NHL 的 BMA 标本。在 305 例（86.1%）中，两种检查结果一致。在 49 例（14%）中发现了不一致：在这 33 例（总人群的 9.3%）中，BMA 的 FC 分析为阳性，而 BMB 经 IHC 支持为阴性；在 16 例（总人群的 4.5%）中，FC 未检测到淋巴细胞浸润，而 BMB 为阳性。尽管这种观察结果的临床意义尚不清楚，但我们认为我们的结果可能对当前的分期程序有用，并为这些患者的微小可测量疾病的设定开辟了一个可能的未来前景。