Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan 650021, People's Republic of China.
BGI Genomics, BGI-Shenzhen, Shenzhen, Guangdong, 518083, People's Republic of China.
Genomics. 2020 Mar;112(2):2028-2033. doi: 10.1016/j.ygeno.2019.11.015. Epub 2019 Nov 21.
Tobacco (Nicotiana tabacum L.) is an essential commercial crop and an ideal model plant for biological mechanism studies. As an allopolyploid species, tobacco harbors a massive and complex genome, which makes the application of molecular markers complicated and challenging. In our study, we performed whole-genome sequencing of an intraspecific recombinant inbred line (RIL) population, a F1 generation and their parents. With the Nicotiana tabacum (K326 cultivar) genome as reference, a total of 45,081 markers were characterized to construct the genetic map, which spanned a genetic distance of 3486.78 cM. Evaluation of a two-dimensional heat map proved the high quality of the genetic map. We utilized these markers to anchor scaffolds and analyzed the ancestral genome origin of linkage groups (LGs). Furthermore, such a high-density genetic map will be applied for quantitative trait locus (QTL) detection, gene localization, genome-wide association studies (GWAS), and marker-assisted breeding in tobacco.
烟草（Nicotiana tabacum L.）是一种重要的商业作物，也是生物机制研究的理想模式植物。作为一个异源多倍体物种，烟草拥有庞大而复杂的基因组，这使得分子标记的应用变得复杂和具有挑战性。在我们的研究中，我们对一个种内重组自交系（RIL）群体、一个 F1 代及其亲本进行了全基因组测序。以 Nicotiana tabacum（K326 品种）基因组为参考，共鉴定了 45081 个标记用于构建遗传图谱，遗传距离为 3486.78 cM。二维热图的评估证明了遗传图谱的高质量。我们利用这些标记来锚定支架，并分析连锁群（LGs）的祖先基因组起源。此外，这种高密度遗传图谱将应用于烟草的数量性状位点（QTL）检测、基因定位、全基因组关联研究（GWAS）和标记辅助育种。
