Departamento de Química Analítica, Instituto Universitario de Investigación en Química Fina y Nanoquímica IUNAN, Universidad de Córdoba, Campus de Rabanales, Edificio Marie Curie (anexo), E-14071, Córdoba, Spain.
Laboratorio de Electroquímica Del Medio Ambiente, LEQMA, Facultad de Química y Biología, Universidad de Santiago de Chile, USACh, Casilla 40, Correo 33, Santiago, Chile.
Anal Chim Acta. 2020 Jan 15;1094:47-56. doi: 10.1016/j.aca.2019.10.021. Epub 2019 Oct 15.
A new planar sorptive phase based on the simple immobilization of polymeric ionic liquids on paper is proposed. The sorptive phase can develop hydrophobic or mixed-mode (combining hydrophobic and ion exchange) interactions with the target analytes. The polymer is prepared by the Radziszewski reaction, which takes place in aqueous media, and it has been thoroughly characterized by different techniques including infrared spectroscopy, matrix-assisted laser desorption/ionization coupled to high-resolution mass spectrometry and proton nuclear magnetic resonance. Three different strategies aimed to immobilize the polymeric ionic liquid on paper have been evaluated. Among them, simple thermal curing at 120 °C was selected. The as-prepared paper has been evaluated for the extraction of several non-steroidal anti-inflammatory drugs from urine, the analytes being finally determined by liquid chromatography with tandem mass spectrometry. The method detection limits were 3.8, 7.2, 6.8, 9.4, 15.7, and 5.1 μg/L for indomethacin, diclofenac, tolmetin, ketoprofen, naproxen, and ibuprofen, respectively. Calibration models were linear (R > 0.9949) up to 1000 μg/L. The intra-day precision, expressed as relative standard deviation and calculated at three different concentrations levels (limit of quantification, 250 μg/L, and 1000 μg/L), varied between 1.1 and 13%. The accuracy, calculated as relative recovery, was in the range from 72 to 95%, thus being considered appropriate. The easiness of polymeric ionic liquid paper synthesis and the multi-sample extraction protocol designed allows the processing of a high number of samples at the same time.
提出了一种基于聚合物离子液体简单固定在纸张上的新型平面吸附相。该吸附相可以与目标分析物形成疏水或混合模式（结合疏水和亲水相互作用）相互作用。聚合物是通过在水介质中发生的 Radziszewski 反应制备的，并通过不同的技术进行了彻底的表征，包括红外光谱、基质辅助激光解吸/电离与高分辨率质谱和质子核磁共振。评估了三种不同的将聚合物离子液体固定在纸张上的策略。其中，选择了简单的 120°C 热固化。评估了制备的纸张从尿液中萃取几种非甾体抗炎药的性能，最终通过液相色谱-串联质谱法测定分析物。方法检测限分别为吲哚美辛、双氯芬酸、托美汀、酮洛芬、萘普生和布洛芬的 3.8、7.2、6.8、9.4、15.7 和 5.1μg/L。校准模型在 1000μg/L 以内呈线性（R>0.9949）。日内精密度，以相对标准偏差表示，在三个不同浓度水平（定量限、250μg/L 和 1000μg/L）计算，变化范围为 1.1-13%。准确度，以相对回收率表示，在 72-95%范围内，因此被认为是合适的。聚合物离子液体纸的合成简单，设计的多样品萃取方案允许同时处理大量样品。
