Affordable and Sustainable Sample Preparation (AS2P) Research Group, Departamento de Química Analítica, Instituto Universitario de Investigación en Química Fina Y Nanoquímica (IUNAN), Universidad de Córdoba, Campus de Rabanales, Edificio Marie Curie, 14071, Córdoba, Spain.
Anal Bioanal Chem. 2022 Jul;414(18):5287-5296. doi: 10.1007/s00216-022-03977-w. Epub 2022 Mar 10.
This article deepens the potential of pre-cleaned bare wooden toothpicks (pb-WTs) for extracting drugs (antidepressants and acetaminophen) from oral fluid samples. The leaching of the intrinsic compounds from the wood matrix is identified as the main challenge for the final determination of the targets, even when a very selective instrumental technique, such as mass spectrometry, is employed. The pre-cleaning of the WTs is proposed for improving the analytical performance. The number of cleaning cycles depends on the injection mode (direct infusion or chromatography) into the mass spectrometer. The different variables affecting the extraction of selected antidepressant drugs were studied in detail, and the optimum procedure was validated using the two mentioned injection modes. The limits of detection were in the ranges 0.1-0.5 ng/mL and 0.1-0.3 ng/mL for direct infusion and liquid chromatography, respectively. The intra-day precision (expressed as relative standard deviation) was better than 12.1% and 8.6%, for direct infusion and liquid chromatography, respectively. Single-blind samples were used to study the applicability of the method. Finally, as a proof-of-concept, the potential of pb-WTs for in vivo sampling was outlined.
本文深化了预处理裸木牙签（pb-WTs）从口腔液样本中提取药物（抗抑郁药和对乙酰氨基酚）的潜力。即使采用非常选择性的仪器技术，如质谱法，从木材基质中浸出内在化合物仍是最终确定目标的主要挑战。建议对 WTs 进行预处理以改善分析性能。清洗循环的次数取决于注入质谱仪的模式（直接注入或色谱法）。详细研究了影响所选抗抑郁药提取的不同变量，并使用上述两种注入模式对最佳程序进行了验证。直接注入和液相色谱的检测限分别在 0.1-0.5 ng/mL 和 0.1-0.3 ng/mL 范围内。直接注入和液相色谱的日内精密度（表示为相对标准偏差）分别优于 12.1%和 8.6%。使用单盲样本研究了该方法的适用性。最后，作为概念验证，概述了 pb-WTs 用于体内采样的潜力。
