Experiment Center of Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China; School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
Innovation Center of Traditional Chinese Medicine Health Service, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
Anal Chim Acta. 2020 Jan 15;1094:70-79. doi: 10.1016/j.aca.2019.09.076. Epub 2019 Oct 3.
To explore how hypochlorous acid (HClO) affects human health, a highly sensitive, selective, and trace detection method for hypochlorite (ClO) is crucial for determining its non-negligible function in both environment and living systems. Herein, a dicyanoisophorone-phenylboronic acid-based novel ratiometric near-infrared fluorescent probe (Probe 1) was designed for the rapid and specific detection of ClO based on the intramolecular charge transfer (ICT) mechanism. Excess addition of HClO to the Probe 1 solution, 186-times ratio (I/I) augment were gained. And this probe provided a colorimetric and ratiometric fluorescence response to ClO with a high selectivity, a rapid response (within 30 s), and had an extremely low detection limit (15.7 nM). In addition, owing to the good sensing properties and low cytotoxicity of Probe 1, it can be used to expediently visualize exogenous ClO in HepG2 cells and endogenous ClO in RAW264.7 macrophage cells. Furthermore, the probe was successfully used for the bioimaging of zebrafish with an acute inflammation. Thus, Probe 1 is a promising vehicle to identify the level of HClO in animals with associated diseases.
为了探索次氯酸（HClO）如何影响人类健康，对于确定次氯酸盐（ClO）在环境和生命系统中不可忽视的功能，开发一种高灵敏度、选择性和痕量检测方法对于 ClO 至关重要。在此，基于分子内电荷转移（ICT）机制，我们设计了一种基于二氰基异佛尔酮-苯硼酸的新型比率型近红外荧光探针（探针 1），用于快速和特异性检测 ClO。向探针 1 溶液中过量添加 HClO，可获得 186 倍的比率（I/I）增强。该探针对 ClO 具有高选择性、快速响应（在 30s 内）和极低的检测限（15.7nM），提供了比色和比率荧光响应。此外，由于探针 1 具有良好的传感性能和低细胞毒性，它可以用于方便地可视化 HepG2 细胞中外源 ClO 和 RAW264.7 巨噬细胞内源性 ClO。此外，该探针成功地用于急性炎症斑马鱼的生物成像。因此，探针 1 是一种很有前途的工具，可以识别相关疾病动物中 HClO 的水平。
