Al-Banna Luma, Sadder Monther T, Lafi Hamzeh A, Dawabah Ahmed A M, Al-Nadhari Saleh N
Department of Plant Protection, Faculty of Agriculture, The University of Jordan, Amman, Jordan.
Department of Horticulture and Crop Science, Faculty of Agriculture, The University of Jordan, Amman, Jordan.
Saudi J Biol Sci. 2019 Nov;26(7):1463-1467. doi: 10.1016/j.sjbs.2018.06.005. Epub 2018 Jun 27.
Ubiquitin expression protein DNA clone (Hl-UBI) was isolated from collected from North Jordan. Its sequence of 285 nucleotides was also determined and deposited in the GeneBank. The 285-bp open reading frame coded for 76-amino acid protein having two domains; the ubiquitin domain and the C terminal extension. The first 59 amino acids were predicted with the ubiquitin domain with identity percentages of 78% to ubiquitin of , 77% to polyubiquitin of , 74% to ubiquitin of and 72% to ubiquitin of . The other domain at the C-terminus, containing 17 amino acids, showed no homology to any known protein. Sequence analysis showed a calculated encoding amino acids molecular weight of 8.77 kDa, theoretical isoelectric point = 4.76, negatively charged residues = 12, positively charged residues = 9, extinction coefficient = 1490, estimated half-life = 30 h, instability index = 32.51 and grand average of hydropathicity = -0.537. The demonstrated subcellular localization analysis of cytoplasm, cell nucleus, mitochondrion, cell skeleton and plasma membrane of Hl-UBI protein occupied about 52.20, 21.70, 17.40, 4.30 and 4.30%, respectively. Sequence, homology and structural analysis confirmed that Hl-UBI gene was highly conserved during evolution and belonged to ubiquitin gene family.
泛素表达蛋白DNA克隆（Hl - UBI）是从约旦北部采集的样本中分离得到的。其285个核苷酸的序列也已测定并提交至基因库。285 bp的开放阅读框编码一个含76个氨基酸的蛋白质，该蛋白质有两个结构域：泛素结构域和C末端延伸区。前59个氨基酸被预测为泛素结构域，与[具体物种1]的泛素同一性百分比为78%，与[具体物种2]的多聚泛素同一性百分比为77%，与[具体物种3]的泛素同一性百分比为74%，与[具体物种4]的泛素同一性百分比为72%。C末端的另一个结构域包含17个氨基酸，与任何已知蛋白质均无同源性。序列分析显示，该编码氨基酸的计算分子量为8.77 kDa，理论等电点 = 4.76，带负电荷的残基 = 12，带正电荷的残基 = 9，消光系数 = 1490，估计半衰期 = 30 h，不稳定指数 = 32.51，亲水性总平均值 = -0.537。对Hl - UBI蛋白进行的亚细胞定位分析表明，其在细胞质、细胞核、线粒体、细胞骨架和质膜中的定位分别约占52.20%、21.70%、17.40%、4.30%和4.30%。序列、同源性和结构分析证实，Hl - UBI基因在进化过程中高度保守，属于泛素基因家族。
