Chen Changlong, Cui Lei, Chen Yongpan, Zhang Hongjun, Liu Pei, Wu Peipei, Qiu Dan, Zou Jingwei, Yang Dan, Yang Li, Liu Hongwei, Zhou Yang, Li Hongjie
The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Department of Plant Pathology, China Agricultural University, Beijing, 100193, China.
Sci Rep. 2017 Nov 3;7(1):14471. doi: 10.1038/s41598-017-14047-y.
Cereal cyst nematode (Heterodera avenae) is attracted to and aggregated around wheat roots to initiate infection, but this interaction between wheat and the nematode is not fully understood. The transcriptional responses of both wheat and H. avenae were examined during their early contact stage by mRNA sequencing analysis; certain numbers of the differentially expressed genes (DEGs) were validated using quantitative real-time PCR. The immobile host wheat root only had 93 DEGs (27 up-regulated and 66 down-regulated), while the mobile plant parasitic nematode reacted much more actively with 879 DEGs (867 up-regulated and 12 down-regulated). Among them, a number of wheat DEGs (mostly down-regulated) were involved in biotic stress pathways, while several putative effector genes were up-regulated in the nematode DEGs. One putative chitinase-like effector gene of H. avenae was able to suppress BAX-triggered programmed cell death in Nicotiana benthamiana. Results of these experiments demonstrated that nematode responded more actively than wheat during the contact stage of parasitism. The parasite's responses mainly involved up-regulation of genes including at least one anti-plant-defence effector gene, whereas the host responses mainly involved down-regulation of certain defence-related genes.
谷类孢囊线虫（燕麦孢囊线虫）会被小麦根系吸引并聚集在其周围以引发感染，但小麦与该线虫之间的这种相互作用尚未完全明晰。通过mRNA测序分析，研究了小麦和燕麦孢囊线虫在早期接触阶段的转录反应；使用定量实时PCR对一定数量的差异表达基因（DEG）进行了验证。不能移动的宿主小麦根系仅有93个DEG（27个上调和66个下调），而可移动的植物寄生线虫反应更为活跃，有879个DEG（867个上调和12个下调）。其中，一些小麦DEG（大多下调）参与生物胁迫途径，而一些假定的效应基因在该线虫的DEG中上调。燕麦孢囊线虫的一个假定几丁质酶样效应基因能够抑制烟草中BAX引发的程序性细胞死亡。这些实验结果表明，在寄生的接触阶段，线虫比小麦反应更活跃。寄生虫的反应主要涉及包括至少一个抗植物防御效应基因在内的基因上调，而宿主的反应主要涉及某些防御相关基因的下调。
