足部组织缺损患者的骨和肌腱培养结果是否一致？

Is there concordance between bone and tendon cultures in patients with foot tissue loss?

作者信息

Dos Santos Vanessa Prado, Alves Carlos Alberto Silveira, Queiroz André Brito, Barberino Maria Goreth Matos de Andrade, Fidelis Ronald José Ribeiro, Fidelis Cícero, de Araújo José Siqueira

机构信息

Universidade Federal da Bahia - UFBA, Instituto de Humanidades Artes e Ciências Professor Milton Santos, Salvador, BA, Brasil.

Universidade Federal da Bahia - UFBA, Complexo Hospitalar Universitário Professor Edgard Santos, Salvador, BA, Brasil.

出版信息

J Vasc Bras. 2019 Nov 8;18:e20190063. doi: 10.1590/1677-5449.190063.

DOI:10.1590/1677-5449.190063

PMID:31762776

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6852700/

Abstract

BACKGROUND

Deep infections of the extremities are a challenge that threaten limb salvage.

OBJECTIVES

To investigate whether the results of bone and deep tissue cultures from patients with trophic limb ulcers coincide.

METHODS

A retrospective study was conducted with data from 54 patients with deep trophic limb ulcers admitted to the Complexo Hospitalar Universitário Professor Edgard Santos, Salvador (BA), Brazil. The study analyzed all patients for whom cultures of material from foot wounds in patients with tissue loss had been performed using two specimen types: bone and fragments of deep tendon. The study analyzed concordance between the two sample types and total number of microorganisms and numbers of microorganisms by Gram staining in both samples.

RESULTS

The mean age of the 54 patients in the sample was 63.6 years, 80% had PAOD, 70% were diabetic, and 72% were hypertensive. Analysis of the cultures showed that 28 (52%) pairs of samples from the 54 patients exhibited complete concordance, with the same microorganisms grown from fragments of deep tendon and bone. There was partial disagreement in 13 samples (24%) and total disagreement in 13 (24%). On average, 1.62 microorganisms were isolated from deep tendon fragments and 1.72 were isolated from bone samples. Analyzing Gram-positive microorganisms separately, the mean number of species grown was 0.48 for tendon cultures and 0.44 for bone cultures. In contrast, the mean number of Gram-negative microorganisms isolated was 1.14 for tendon samples and 1.27 for bone samples.

CONCLUSIONS

Around half of the patients with foot tissue loss had bone and tendon cultures that coincided exactly.

摘要

背景

肢体深部感染是一项威胁肢体保全的挑战。

目的

研究营养性肢体溃疡患者的骨组织培养结果与深部组织培养结果是否一致。

方法

对巴西萨尔瓦多市（巴伊亚州）埃德加德·桑托斯教授大学综合医院收治的54例营养性肢体深部溃疡患者的数据进行回顾性研究。该研究分析了所有使用两种标本类型（骨组织和深部肌腱碎片）对组织缺损患者足部伤口材料进行培养的患者。研究分析了两种样本类型之间的一致性、微生物总数以及两种样本中革兰氏染色的微生物数量。

结果

样本中54例患者的平均年龄为63.6岁，80%患有外周动脉疾病，70%患有糖尿病，72%患有高血压。培养分析显示，54例患者中有28对样本（52%）表现出完全一致，深部肌腱碎片和骨组织培养出相同的微生物。13个样本（24%）存在部分不一致，13个样本（24%）完全不一致。平均而言，从深部肌腱碎片中分离出1.62种微生物，从骨组织样本中分离出1.72种微生物。分别分析革兰氏阳性微生物，肌腱培养物中生长的平均菌种数为0.48种，骨组织培养物中为0.44种。相比之下，肌腱样本中分离出的革兰氏阴性微生物平均数量为1.14种，骨组织样本中为1.27种。