Hua Shiyao, Liu Jiayue, Zhang Yiwei, Li Juan, Zhang Xinhui, Dong Lin, Zhao Yunsheng, Fu Xueyan
School of Pharmacy Ningxia Medical University Yinchuan China.
School of Basic Medical Sciences Ningxia Medical University Yinchuan China.
Food Sci Nutr. 2019 Sep 10;7(11):3443-3451. doi: 10.1002/fsn3.1185. eCollection 2019 Nov.
Long pepper ( L.) and black pepper ( L.) plants are commonly used as spices around the world and have also been postulated to have medicinal effects. Piperine, as the major alkaloid of and , has gained wide attention of the medical community and culinary enthusiasts. This study seeks to determine the effects of piperine on neuronal apoptosis in peri-infarcted cerebral cortices of rats with permanent middle cerebral artery occlusion (pMCAO) injury. Evaluation of the different behavioral components was conducted after pMCAO. 2, 3, 5-Triphenyltetrazolium chloride (TTC) was used to evaluate the area of cortical ischemia. Gross histopathological changes, as well as microscopic neuronal changes, were observed in brain tissue samples. The protein expression of Caspase-3, Caspase-9, Bax, Bcl-2, and Cytochrome C (Cyt-c) was analyzed using western blotting. The findings reveal that rats that received piperine treatment show markedly decreased neurological deficit, less ischemia-induced cellular damage, as well as smaller areas of cerebral infarction, with less severe macro and microcellular cerebral structural changes. Western blotting analysis reveals that piperine administration inhibits Bax, while enhancing Bcl-2 expression. The protein expression of Caspase-3, Caspase-9, and Cyt-c was also found to be significantly inhibited. We conclude that piperine may provide several beneficial neuroprotective effects that warrant further investigation.
荜茇和黑胡椒在世界各地普遍用作香料，并且据推测还具有药用功效。胡椒碱作为荜茇和黑胡椒的主要生物碱，已引起医学界和烹饪爱好者的广泛关注。本研究旨在确定胡椒碱对永久性大脑中动脉闭塞（pMCAO）损伤大鼠梗死周围大脑皮质神经元凋亡的影响。在pMCAO后对不同行为成分进行评估。使用2,3,5-三苯基氯化四氮唑（TTC）评估皮质缺血面积。观察脑组织样本中的大体组织病理学变化以及微观神经元变化。使用蛋白质印迹法分析半胱天冬酶-3（Caspase-3）、半胱天冬酶-9（Caspase-9）、Bax、Bcl-2和细胞色素C（Cyt-c）的蛋白表达。研究结果显示，接受胡椒碱治疗的大鼠神经功能缺损明显减轻，缺血诱导的细胞损伤减少，脑梗死面积更小，大脑宏观和微观细胞结构变化也较轻。蛋白质印迹分析显示，给予胡椒碱可抑制Bax，同时增强Bcl-2表达。还发现Caspase-3、Caspase-9和Cyt-c的蛋白表达受到显著抑制。我们得出结论，胡椒碱可能具有多种有益的神经保护作用，值得进一步研究。
