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基于等压标签相对和绝对定量的小麦中真菌病原体引发的防御反应的蛋白质组学分析数据。

The data of Isobaric tags for relative and absolute quantification-based proteomic analysis of defense responses triggered by the fungal pathogen in wheat.

作者信息

Wang Biao, Li Xuefeng, Chen Wuying, Kong Lingrang

机构信息

State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian, 271018, China.

出版信息

Data Brief. 2019 Nov 2;27:104747. doi: 10.1016/j.dib.2019.104747. eCollection 2019 Dec.

DOI:10.1016/j.dib.2019.104747
PMID:31763403
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6864187/
Abstract

Fusarium head blight (FHB) is one of the most prevalent diseases of wheat and other small grain cereals that is predominantly caused by the fungal pathogen . Extraction of total proteins were from tissues of A061-3 and A061-4 plants. Three biological replicates were carried out for each line at four time points. Samples were performed using iTRAQ (Isobaric tags for relative and absolute quantification). This data is being made available to increase the understanding of FHB resistance proteomics. The data from this study are related to the research article "Isobaric tags for relative and absolute quantification-based proteomic analysis of defense responses triggered by the fungal pathogen in wheat" [1].

摘要

镰刀菌穗腐病(FHB)是小麦和其他小粒谷物中最普遍的病害之一,主要由真菌病原体引起。从A061 - 3和A061 - 4植株的组织中提取总蛋白。每个品系在四个时间点进行了三次生物学重复。使用iTRAQ(相对和绝对定量等压标签)进行样本分析。提供这些数据是为了增进对FHB抗性蛋白质组学的理解。本研究的数据与研究论文《基于相对和绝对定量等压标签的小麦中真菌病原体触发的防御反应的蛋白质组学分析》[1]相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92c8/6864187/c8bed28da8ba/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92c8/6864187/7c2cb352c13c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92c8/6864187/c953b5f5b497/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92c8/6864187/c8bed28da8ba/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92c8/6864187/7c2cb352c13c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92c8/6864187/c953b5f5b497/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92c8/6864187/c8bed28da8ba/gr3.jpg

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本文引用的文献

1
Isobaric tags for relative and absolute quantification-based proteomic analysis of defense responses triggered by the fungal pathogen Fusarium graminearum in wheat.基于相对和绝对定量的同位标签蛋白质组学分析真菌病原体禾谷镰刀菌诱导的小麦防御反应。
J Proteomics. 2019 Sep 15;207:103442. doi: 10.1016/j.jprot.2019.103442. Epub 2019 Jul 19.
2
iTRAQ-based quantitative proteomics analysis of rice leaves infected by Rice stripe virus reveals several proteins involved in symptom formation.基于iTRAQ的水稻条纹病毒感染水稻叶片的定量蛋白质组学分析揭示了几种参与症状形成的蛋白质。
Virol J. 2015 Jun 26;12:99. doi: 10.1186/s12985-015-0328-y.
3
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
一种利用蛋白质 - 染料结合原理对微克级蛋白质进行定量的快速灵敏方法。
Anal Biochem. 1976 May 7;72:248-54. doi: 10.1016/0003-2697(76)90527-3.