Pochanagone Suttahatai, Rittiron Ronnarit
Department of Food Engineering, Faculty of Engineering at Kamphaeng Saen, Kasetsart University, Nakhonpathom 73140, Thailand.
ACS Omega. 2019 Nov 6;4(21):19164-19171. doi: 10.1021/acsomega.9b02438. eCollection 2019 Nov 19.
Rapid and simple methods to determine histamine in tuna fish have been examined. A dry extract system for infrared (DESIR) was coupled with near-infrared spectroscopy in order to obtain the absorption of histamine in tuna fish at the ppm level. The result showed that the optimal extraction solvent for preparing DESIR samples was 75% methanol and boiling water (100 °C). Calibration equations were developed and tested by independent validation set samples. The calibration equation developed from boiling water as solvent extraction was slightly better than the equation developed from 75% methanol solvent with a coefficient of determination ( ) of 0.79, a standard error of calibration of 2.45 ppm, a standard error of prediction of 2.94 ppm, and a bias of 0.10 ppm. Furthermore, the predicted values from both equations were not significantly different from the reference values obtained from the standard method at the 95% confidence interval. Compared to the current AOAC fluorometric official method, the proposed technique simplified and reduced the preparation time.
已对测定金枪鱼中组胺的快速简便方法进行了研究。将红外干燥萃取系统（DESIR）与近红外光谱联用，以获取金枪鱼中百万分之一级组胺的吸收情况。结果表明，制备DESIR样品的最佳萃取溶剂是75%甲醇和沸水（100℃）。通过独立验证集样品建立并测试了校准方程。以沸水作为溶剂萃取建立的校准方程略优于以75%甲醇溶剂建立的方程，其决定系数（ ）为0.79,校准标准误差为2.45 ppm，预测标准误差为2.94 ppm，偏差为0.10 ppm。此外，在95%置信区间内，两个方程的预测值与标准方法获得的参考值无显著差异。与现行美国官方分析化学师协会荧光法定方法相比，所提出的技术简化并缩短了制备时间。
