Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, Guangdong Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Department of Clinical Laboratory, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Biosci Rep. 2019 Dec 20;39(12). doi: 10.1042/BSR20191087.
Dysregulation of microRNAs (miRNAs) contributes to tumor progression via the regulation of the expression of specific oncogenes and tumor suppressor genes. One such example, miR-27b-3p, has reportedly been involved in tumor progression in many types of cancer. The aim of the present study was to delve into the role and the underlying mechanism of miR-27b-3p in colorectal cancer (CRC) cells.
In the present study, we detected the expression level of miR-27b-3p by RT-PCR. The effect of miR-27b-3p overexpression on cell proliferation in CRC cells was evaluated by cell counting and Edu assays. Transwell migration and invasion assays were used to examine the effects of cell migration and invasion. Bioinformatics, luciferase reporter assay and western blot assay were performed to identify the target of miR-27b-3p.
Here, we have demonstrated that although miR-27b-3p can affect cell morphology, it has no observable effect on the proliferation of CRC cells. However, it significantly promotes the migration and invasion of CRC cells. We discovered that HOXA10 was a newly identified target of miR-27b-3p in CRC cells, as confirmed by bioinformatics, western blots and dual luciferase reporter assay. Furthermore, the overexpression of miR-27b-3p or the suppression of HOXA10 can activate the integrin β1 signaling pathway. In conclusion, our results reveal a new function of miR-27b-3p that demonstrates its ability to promote CRC cell migration and invasion by targeting the HOXA10/integrin β1 cell signal axis.
This may provide a mechanism to explain why miR-27b-3p promotes CRC cell migration and invasion.
microRNAs（miRNAs）的失调通过调节特定癌基因和肿瘤抑制基因的表达促进肿瘤进展。miR-27b-3p 就是这样一个例子，据报道它参与了多种癌症的肿瘤进展。本研究旨在深入研究 miR-27b-3p 在结直肠癌（CRC）细胞中的作用和潜在机制。
在本研究中，我们通过 RT-PCR 检测 miR-27b-3p 的表达水平。通过细胞计数和 Edu 检测评估 miR-27b-3p 过表达对 CRC 细胞增殖的影响。Transwell 迁移和侵袭实验用于检测细胞迁移和侵袭的影响。生物信息学、荧光素酶报告基因检测和 Western blot 检测用于鉴定 miR-27b-3p 的靶标。
在这里，我们已经证明，尽管 miR-27b-3p 可以影响细胞形态，但对 CRC 细胞的增殖没有明显影响。然而，它显著促进了 CRC 细胞的迁移和侵袭。我们发现，HOXA10 是 CRC 细胞中 miR-27b-3p 的一个新的靶标，这一点通过生物信息学、Western blot 和双荧光素酶报告基因检测得到了证实。此外，miR-27b-3p 的过表达或 HOXA10 的抑制可以激活整合素 β1 信号通路。总之，我们的结果揭示了 miR-27b-3p 的一个新功能，即通过靶向 HOXA10/整合素 β1 细胞信号轴促进 CRC 细胞迁移和侵袭。
这可能为解释为什么 miR-27b-3p 促进 CRC 细胞迁移和侵袭提供了一种机制。
