Meng Xiangyun, Gong Kun, Sun Chao, Liu Dianwei, Du Pinggong, Xu Dapeng
Department of Oral and Maxillofacial Surgery, Yantai Stomatological Hospital Affiliated to Binzhou Medical College, Yantai, China.
J Craniofac Surg. 2020 Mar/Apr;31(2):416-419. doi: 10.1097/SCS.0000000000006020.
In this study, nonmineralized silk fibroin/gelatin (SF/G) hybrid scaffolds and the mineralized SF/G/hydroxyapatite (SF/G/HA) hybrid scaffolds were fabricated using vacuum freeze-drying method and biomineralization technique. The morphology and mechanical properties of the 2 hybrid scaffolds were characterized. Furthermore, the cytotoxic profiles of the hybrid scaffolds were investigated in vitro by seeding the human osteoblast cells (hFOB1.19). The 2 hybrid scaffolds were both highly porous and the pore sizes of the SF/G as well as SF/G/HA hybrid scaffolds were 260 ± 58 μm and 210 ± 35 μm, respectively. Compared with the SF/G hybrid scaffold, the SF/G/HA hybrid scaffolds exhibited significantly enhanced compressive strength and modulus. Significant early cell adhesion and proliferation on the SF/G hybrid scaffolds were observed, while SF/G/HA hybrid scaffolds effectively improved osteogenic differentiation of hFOB1.19 after 10 days of coculture. The results confirmed that the 2 hybrid scaffolds were both cytocompatible and had almost no negative effects on the hFOB1.19 in vitro. However, the SF/G/HA hybrid scaffolds tended to be more promising for application in bone-tissue engineering with good mechanical property and osteogenic differentiation.
在本研究中，采用真空冷冻干燥法和生物矿化技术制备了非矿化丝素蛋白/明胶（SF/G）复合支架和矿化的SF/G/羟基磷灰石（SF/G/HA）复合支架。对这两种复合支架的形态和力学性能进行了表征。此外，通过接种人成骨细胞（hFOB1.19）在体外研究了复合支架的细胞毒性特征。两种复合支架均具有高度多孔性，SF/G复合支架和SF/G/HA复合支架的孔径分别为260±58μm和210±35μm。与SF/G复合支架相比，SF/G/HA复合支架的抗压强度和模量显著提高。观察到SF/G复合支架上有明显的早期细胞黏附和增殖，而在共培养10天后，SF/G/HA复合支架有效地促进了hFOB1.19的成骨分化。结果证实，这两种复合支架均具有细胞相容性，且在体外对hFOB1.19几乎没有负面影响。然而，SF/G/HA复合支架因其良好的力学性能和成骨分化能力，在骨组织工程应用中更具前景。
