Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Phatumwan, Bangkok 10330, Thailand.
J Biomed Mater Res B Appl Biomater. 2012 Nov;100(8):2307-15. doi: 10.1002/jbm.b.32802. Epub 2012 Sep 26.
In this study, the surface modification of silk fibroin (SF) scaffolds with gelatin/chitooligosaccharide (G/COS) blends using the reaction of glutaraldehyde (GA) was established. The effects of G/COS mixing ratio (100/0, 90/10, 80/20, and 70/30) and GA crosslinking concentration (0.05, 0.10, 0.15, and 0.20 vol %) on the properties of scaffolds were investigated. At 0.10-0.20 vol % GA, all G/COS blends could be successfully conjugated on the SF scaffolds, as confirmed by the percentage of weight increased and the presence of functional groups indicating SF, G, and COS from FTIR spectra. Pore size of SF scaffolds was around 570 μm with 92% porosity, however, the G/COS-conjugated SF scaffolds showed thickened pore's wall, smaller pore size (∼184-275 μm) and less porosity (∼81%), but increased density. This modified structure subsequently improved the compressive modulus of the G/COS-conjugated SF scaffolds. In terms of biological properties, the gelatin-conjugated SF scaffolds promoted the attachment and proliferation of bone marrow-derived mesenchymal stem cells (MSC) rather than the other scaffolds. However, the G/COS-conjugated SF scaffolds, particularly at the ratio of 70/30, promoted the osteogenic differentiation of MSC comparing to the SF scaffold, as confirmed by the production of alkaline phosphatase (ALP) activity and calcium (Ca), and the deposition of calcium phosphate (CaP). It was concluded that the G/COS-conjugated SF scaffolds showed great mechanical properties due to the β-structure of silk fibroin, as well as the enhanced biological properties due to the G/COS blends.
在这项研究中，使用戊二醛（GA）的反应，成功地在丝素蛋白（SF）支架表面修饰了明胶/壳寡糖（G/COS）共混物。研究了 G/COS 混合比（100/0、90/10、80/20 和 70/30）和 GA 交联浓度（0.05、0.10、0.15 和 0.20 体积%）对支架性能的影响。在 0.10-0.20 体积%GA 下，所有 G/COS 共混物都可以成功地接枝到 SF 支架上，这可以通过增加的重量百分比和表明 SF、G 和 COS 的功能基团从 FTIR 光谱来证实。SF 支架的孔径约为 570μm，孔隙率为 92%，然而，G/COS 接枝 SF 支架显示出增厚的孔壁、更小的孔径（约 184-275μm）和较低的孔隙率（约 81%），但密度增加。这种改性结构随后提高了 G/COS 接枝 SF 支架的压缩模量。在生物性能方面，与其他支架相比，明胶接枝 SF 支架促进了骨髓间充质干细胞（MSC）的附着和增殖。然而，与 SF 支架相比，G/COS 接枝 SF 支架特别在 70/30 的比例下，促进了 MSC 的成骨分化，这可以通过碱性磷酸酶（ALP）活性和钙（Ca）的产生以及磷酸钙（CaP）的沉积来证实。研究结论认为，G/COS 接枝 SF 支架由于丝素蛋白的β结构而表现出良好的机械性能，并且由于 G/COS 共混物而增强了生物性能。
