Bol S, Visser J, van den Engh G
Exp Hematol. 1979 Nov;7(10):541-53.
The heterogeneity among progenitor cells of granulocytes and macrophages capable of forming colonies in vitro (CFU-c) is analysed. If specific culture conditions and stimuli are used, it is possible to discriminate among three subpopulations of CFU-c. CFU-c directly responding to CSF prepared from pregnant mouse uteri are characterized by a density of 1.075 g.cm-3. The G1-phase cells of this CFU-c subpopulation have a sedimentation rate of 4.8 mm.h-1. CFU-c of two additional subpopulations are induced to proliferate by enhancing factors present in serum of mice injected with endotoxin 18 hours earlier and in lysate of rat erythrocytes. CFU-c responding to postendotoxin serum have a density of 1.070 g.cm-3 and a sedimentation rate of 4.3 mm.h-1 (G1 cells). The CFU-c responding to erythrocyte lysate have a density of 1.080 g.cm-3 and a sedimentation rate of 5.3 mm.h-1 (G1 cells). The calculated diameters of G1-phase cells of these three CFU-c subpopulations range from 7.4 to 7.6 micron and are not significantly different. It is proposed that the three CFU-c detected represent three sequential maturation stages of granulocyte/macrophage progenitor cells. These cells are similar in size but increase in density (1.070 g.cm-3 to 1.080 g.cm-3) as they mature.
对体外能够形成集落的粒细胞和巨噬细胞祖细胞（CFU-c）之间的异质性进行了分析。如果使用特定的培养条件和刺激因素，就有可能区分CFU-c的三个亚群。直接对从怀孕小鼠子宫制备的集落刺激因子（CSF）作出反应的CFU-c，其特征密度为1.075 g.cm-3。该CFU-c亚群的G1期细胞沉降速率为4.8 mm.h-1。另外两个亚群的CFU-c可被提前18小时注射内毒素的小鼠血清中存在的增强因子以及大鼠红细胞裂解物诱导增殖。对内毒素后血清作出反应的CFU-c密度为1.070 g.cm-3，沉降速率为4.3 mm.h-1（G1期细胞）。对红细胞裂解物作出反应的CFU-c密度为1.080 g.cm-3，沉降速率为5.3 mm.h-1（G1期细胞）。这三个CFU-c亚群的G1期细胞计算直径在7.4至7.6微米之间，且无显著差异。有人提出，检测到的这三个CFU-c代表粒细胞/巨噬细胞祖细胞的三个连续成熟阶段。这些细胞大小相似，但随着成熟其密度增加（从1.070 g.cm-3至1.080 g.cm-3）。
