Byrne P V, Heit W, Kubanek B
Exp Hematol. 1979 Feb;7(2):105-12.
Possible variation in proliferative state among subpopulations of myelopoietic progenitor cells of mouse bone marrow (CFU-c), which form in vitro colonies of granulocytes and/or macrophages, was investigated by estimation of the percentage in S phase of the cell cycle. Subpopulations of CFU-c, obtained by separation of femoral marrow cells on continuous albumin density gradients, were detected by culture in agar with different types of colony stimulating factor (CSF). The percentage of CFU-c in S phase, detected with mouse lung conditioned medium (CSFMLCM), averaged 17%. This was significantly lower than the 41% which was found when an extract of human urine (CSFHU) was used. This result was obtained with unfractionated marrow cells both by in vitro suicide with tritiated thymidine and by in vivo administration of hydroxyurea. There were different CFU-c responses to CSF, on the basis of their density distributions. Those responding to CSFMLCM had a low density disposition whereas the CSFHU responders had a high density disposition. The administration of hydroxyurea resulted in an alteration in the density distribution which indicated that a larger proportion of high density CFU-c were in S phase. The results indicate that CFU-c responding to different types of CSF differ in buoyant density and proliferative state.
通过估计细胞周期S期的百分比，研究了小鼠骨髓中形成粒细胞和/或巨噬细胞体外集落的骨髓祖细胞亚群（CFU-c）增殖状态的可能差异。通过在连续白蛋白密度梯度上分离股骨骨髓细胞获得CFU-c亚群，在含有不同类型集落刺激因子（CSF）的琼脂中培养来检测。用小鼠肺条件培养基（CSFMLCM）检测到的处于S期的CFU-c百分比平均为17%。这显著低于使用人尿提取物（CSFHU）时发现的41%。无论是通过体外氚标记胸腺嘧啶自杀法还是体内给予羟基脲，未分级的骨髓细胞都得到了这一结果。根据CFU-c的密度分布，它们对CSF有不同的反应。对CSFMLCM有反应的细胞具有低密度分布，而对CSFHU有反应的细胞具有高密度分布。羟基脲的给药导致密度分布发生改变，这表明较大比例的高密度CFU-c处于S期。结果表明，对不同类型CSF有反应的CFU-c在浮力密度和增殖状态上存在差异。
