Placental gas exchange during amniotic carbon dioxide insufflation in sheep.

OBJECTIVE

Insufflation of the amniotic cavity with carbon dioxide (CO ) is used clinically to improve visibility during complex fetoscopic surgery. Insufflation with heated, humidified CO has recently been shown to reduce fetal hypercapnia and acidosis in sheep, compared with use of cold and dry CO , but the underlying mechanisms are unclear. The aim of this study was to investigate whether differences in placental CO and oxygen (O ) exchange during insufflation with heated and humidified vs cold and dry CO could explain these findings.

METHODS

Thirteen fetal lambs at 105 days of gestation (term, 146 days) were exteriorized partially, via a midline laparotomy and hysterotomy, and instrumented with an umbilical artery catheter, an umbilical vein catheter and a common umbilical vein flow probe. Arterial and venous catheters and flow probes were also inserted into the maternal uterine circulation. Six ewes were insufflated with cold, dry CO (22°C; 0-5% humidity) and seven with heated, humidified CO (40°C; 95-100% humidity) at 15 mmHg for 180 min. Blood-flow recordings and paired arterial and venous blood gases were sampled from uterine and umbilical vessels. Rates of placental CO and O exchange were calculated.

RESULTS

After 180 min of insufflation, fetal survival was 33% (2/6) using cold, dry CO and 71% (5/7) using heated, humidified CO . By 120 min, fetuses insufflated with heated, humidified CO had lower arterial CO levels and higher arterial pH compared to those insufflated with cold, dry gas. Insufflation decreased significantly placental gas exchange in both groups, as measured by rates of both (i) fetal CO clearance and O uptake and (ii) maternal O delivery and CO uptake from the fetal compartment.

CONCLUSIONS

Lower arterial CO and higher pH levels in fetuses insufflated with heated and humidified, compared to cold and dry, CO could not be explained by differences in placental gas exchange. Instead, heated and humidified insufflation appeared to reduce fetal CO absorption from the uterus, supporting its use in preference to cold, dry CO . © 2019 International Society of Ultrasound in Obstetrics and Gynecology.