Department of Obstetrics and Gynecology, The Second Hospital of Hebei Medical University, 215 Heping West Road, Shijiazhuang 050000, China.
Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, China.
Int J Biol Macromol. 2020 Jan 15;143:163-172. doi: 10.1016/j.ijbiomac.2019.11.184. Epub 2019 Nov 22.
Herein we report a facile approach for chitosan-heparin hydrogels with controlled release manner and their applications for intrauterine adhesion. The sol precursor was converted to gel at physiological temperature in 15 min. FTIR, SEM and swelling test were performed to characterize their compositions, morphologies and stability. In vitro releasing profiles was investigated in PBS solutions. Intrauterine injured rat model was established and treated with different methods. The results of H&E staining, Masson trichrome staining, western blots assay, immunohistochemical staining and immunofluorescence staining revealed that endogenous c-kit positive stem cells (HSCs) were recruited to the injury site and promoted the wound recovery. After 7 days' treatment, uterus treated with SDF-1α releasing hydrogel showed no difference with control group on endometrial thickness, glands number and fibrosis level. This work provides a possible method for intrauterine adhesion healing.
在此，我们报告了一种简便的壳聚糖-肝素水凝胶制备方法，具有可控的释放方式，并将其应用于宫腔粘连。该溶胶前体在生理温度下 15 分钟内转化为凝胶。通过傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）和溶胀试验对其组成、形态和稳定性进行了表征。在 PBS 溶液中研究了体外释放曲线。建立了宫内损伤大鼠模型，并采用不同方法进行治疗。苏木精-伊红（H&E）染色、Masson 三色染色、western blot 检测、免疫组化染色和免疫荧光染色的结果表明，内源性 c-kit 阳性干细胞（HSCs）被募集到损伤部位，并促进了伤口的恢复。治疗 7 天后，SDF-1α 释放水凝胶处理的子宫在内膜厚度、腺体数量和纤维化程度上与对照组无差异。这项工作为宫腔粘连的愈合提供了一种可能的方法。
