Ge Fei, Jia Congjun, Chu Min, Liang Chunnian, Yan Ping
Key Laboratory of Yak Breeding Engineering Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Science, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China.
Animals (Basel). 2019 Nov 21;9(12):1008. doi: 10.3390/ani9121008.
Copy number variation (CNV) is currently accepted as a common source of genetic variation. It is reported that CNVs may influence the resistance to disease and complex economic traits, such as residual feed intake, muscle formation, and fat deposition in livestock. Cell adhesion molecule 2 (CADM2) is expressed widely in the brain and adipose tissue and can regulate body weight through the central nervous system. Growth traits are important economic traits for animal selection. In this study, we aimed to explore the effect of CADM2 gene copy number variants on yak growth traits. Here, two CNVs in the CADM2 gene were investigated using the quantitative polymerase chain reaction (qPCR), and the association of the CNVs with growth traits in yak was analyzed using statistical methods by SPSS software. Differences were considered significant if the value was < 0.05. Statistical analysis indicated significant association of CADM2-CNV2 with the body weight of the Chinese Ashidan yak. A significant effect of CNV2 ( < 0.05) was found on body weight at 6 months. In CNV2, the gain-type copy number variation exhibited greater performance than the other variants, with greater body weight observed at 6 months ( < 0.05). To the best of our knowledge, this is the first attempt to investigate the function of CADM2-CNVs and their association with growth traits in animals. This may be a useful candidate marker in marker-assisted selection of yaks.
拷贝数变异（CNV）目前被认为是遗传变异的常见来源。据报道，CNV可能会影响家畜对疾病的抵抗力以及复杂的经济性状，如剩余采食量、肌肉形成和脂肪沉积。细胞粘附分子2（CADM2）在大脑和脂肪组织中广泛表达，并可通过中枢神经系统调节体重。生长性状是动物选育中的重要经济性状。在本研究中，我们旨在探究CADM2基因拷贝数变异对牦牛生长性状的影响。在此，我们使用定量聚合酶链反应（qPCR）研究了CADM2基因中的两个CNV，并使用SPSS软件通过统计方法分析了这些CNV与牦牛生长性状的关联。如果P值<0.05，则差异被认为具有统计学意义。统计分析表明，CADM2-CNV2与中国阿什旦牦牛的体重存在显著关联。在6个月龄时，发现CNV2对体重有显著影响（P<0.05）。在CNV2中，增益型拷贝数变异的表现优于其他变异，在6个月龄时观察到体重更大（P<0.05）。据我们所知，这是首次尝试研究CADM2-CNVs的功能及其与动物生长性状的关联。这可能是牦牛标记辅助选择中一个有用的候选标记。
