不同传感器体外单轴加载诱导小鼠胫骨的应变检测。

Detection of the Strains Induced in Murine Tibias by Ex Vivo Uniaxial Loading with Different Sensors.

机构信息

Department of Mechanical and Aerospace Engineering, Sapienza University of Rome, 00184 Rome, Italy.

Multifactorial Disease and Complex Phenotype Research Area, Children's Hospital Bambino Gesù, 00146 Rome, Italy.

出版信息

Sensors (Basel). 2019 Nov 22;19(23):5109. doi: 10.3390/s19235109.

DOI:10.3390/s19235109

PMID:31766596

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6928746/

Abstract

In this paper, the characterization of the main techniques and transducers employed to measure local and global strains induced by uniaxial loading of murine tibiae is presented. Micro strain gauges and digital image correlation (DIC) were tested to measure local strains, while a moving coil motor-based length transducer was employed to measure relative global shortening. Local strain is the crucial parameter to be measured when dealing with bone cell mechanotransduction, so we characterized these techniques in the experimental conditions known to activate cell mechanosensing in vivo. The experimental tests were performed using tibia samples excised from twenty-two C57BL/6 mice. To evaluate measurement repeatability we computed the standard deviation of ten repetitive compressions to the mean value. This value was lower than 3% for micro strain gauges, and in the range of 7%-10% for DIC and the length transducer. The coefficient of variation, i.e., the standard deviation to the mean value, was about 35% for strain gauges and the length transducer, and about 40% for DIC. These results provided a comprehensive characterization of three methodologies for local and global bone strain measurement, suggesting a possible field of application on the basis of their advantages and limitations.

摘要

本文介绍了用于测量单轴加载诱导的鼠胫骨局部和整体应变的主要技术和换能器的特性。微应变计和数字图像相关（DIC）被测试用于测量局部应变，而基于动圈电机的长度换能器用于测量相对整体缩短。局部应变是处理骨细胞机械转导时需要测量的关键参数，因此我们在已知在体内激活细胞机械感觉的实验条件下对这些技术进行了表征。实验测试使用从 22 只 C57BL/6 小鼠中取出的胫骨样本进行。为了评估测量的重复性，我们计算了十次重复压缩的标准偏差与平均值的比值。对于微应变计，该值低于 3%，而对于 DIC 和长度换能器，该值在 7%-10%范围内。应变计和长度换能器的变异系数（即标准偏差与平均值的比值）约为 35%，而 DIC 的变异系数约为 40%。这些结果全面表征了三种用于局部和整体骨应变测量的方法，根据它们的优缺点，为它们的可能应用领域提供了参考。