Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, Sichuan, PR China; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, PR China.
Department of Veterinary Biosciences, Melbourne Veterinary School, The University of Melbourne, Parkville, Victoria, 3010, Australia.
Vet Microbiol. 2019 Dec;239:108481. doi: 10.1016/j.vetmic.2019.108481. Epub 2019 Oct 31.
Multidrug-resistant and hypervirulent Klebsiella pneumoniae (hvKP) poses a significant risk to public health. To better understand the molecular characteristics of multidrug-resistant and hypervirulent K. pneumoniae of animal origin, fifteen K. pneumoniae strains from the liver, blood of sick pigs and chicken feces were collected. All K. pneumoniae isolates were subjected to antimicrobial susceptibility testing, string test, multi-locus sequence typing and whole genome sequencing. Seven K. pneumoniae isolates were found carrying the mcr-1.1 gene. Among them, a multidrug-resistant and hypervirulent K. pneumoniae strain SCsl1 isolated from the liver of a diseased pig was found to harbor 16 resistance genes (e.g., mcr-1.1) and 16 virulence genes including aerobactin. Moreover, a novel integrative and conjugative element, named ICEKpSL1, was identified in SCsl1, which contains a full Yersinia high-pathogenicity island (HPI). This element could be excised from the chromosome to form a circular intermediate, indicating potential transmission of the Yersinia pathogenicity island. The emergence of multidrug-resistance and hypervirulence in K. pneumoniae from animals warrants further surveillance.
多药耐药和高毒力肺炎克雷伯菌（hvKP）对公共卫生构成重大威胁。为了更好地了解动物源多药耐药和高毒力肺炎克雷伯菌的分子特征，从病猪的肝脏、血液和鸡粪便中采集了 15 株肺炎克雷伯菌。对所有肺炎克雷伯菌分离株进行了抗菌药物敏感性试验、string 试验、多位点序列分型和全基因组测序。发现 7 株肺炎克雷伯菌携带 mcr-1.1 基因。其中，从病猪肝脏中分离到一株多药耐药和高毒力肺炎克雷伯菌 SCsl1，携带 16 个耐药基因（如 mcr-1.1）和 16 个毒力基因，包括aerobactin。此外，在 SCsl1 中发现了一个新型的整合和共轭元件，命名为 ICEKpSL1，它包含一个完整的耶尔森氏菌高致病性岛（HPI）。该元件可从染色体上切除，形成一个圆形中间产物，表明耶尔森氏菌致病性岛具有潜在的传播能力。动物源肺炎克雷伯菌的多药耐药和高毒力的出现需要进一步监测。
