Laboratorio de Investigación en Agentes Antibacterianos, Facultad de Ciencias Biológicas, Universidad de Concepción , Concepción, Chile.
Millennium Nucleus for Collaborative Research on Bacterial Resistance , Chile.
Virulence. 2021 Dec;12(1):35-44. doi: 10.1080/21505594.2020.1859274.
Convergence of virulence and antibiotic-resistance has been reported in , but not in . We, hereby, report the detection and genomic characterization of hypervirulent and hypermucoviscous and recovered in Chile from health-care associated infections, which displayed resistance to broad-spectrum cephalosporins. One hundred forty-six complex isolates were screened by hypermucoviscosity by the "string test." Two hypermucoid isolates, one hypermucoviscous (hmKp) and one (hmKv), were further investigated by whole-genome sequencing. virulence was analyzed by the killing assay. analysis of hmKp UCO-494 and hmKv UCO-495 revealed the presence of multiple antibiotic-resistance genes, such as , and among others clinically relevant resistance determinants, including mutations in a two-component regulatory system related to colistin resistance. These genetic features confer a multidrug-resistant (MDR) phenotype in both strains. Moreover, virulome analysis confirmed the presence of the aerobactin gene , in addition to yersiniabactin and/or colicin V encoding genes, which are normally associated to high virulence in humans. Furthermore, both isolates were able to kill and displayed higher virulence in comparison with the control strain. In summary, the convergence of virulence and the MDR-phenotype in complex members is reported for the first time in Chile, denoting a clinical problem that deserves special attention and continuous surveillance in South America.
毒力和抗生素耐药性的趋同已在 中报道，但在 中尚未报道。在此，我们报告了在智利从与医疗保健相关的感染中分离出的超毒力和超粘液 和 的检测和基因组特征，这些菌株对广谱头孢菌素表现出耐药性。通过“string test”对 146 株 复合菌株进行超粘液性筛选。对 2 株粘液性分离株，即超粘液性 （hmKp）和 （hmKv）进行了全基因组测序。通过杀菌试验分析了毒力。hmKp UCO-494 和 hmKv UCO-495 的 分析显示存在多种抗生素耐药基因，如 、 等，以及其他临床相关耐药决定因素，包括与多粘菌素耐药相关的双组分调节系统的突变。这些遗传特征使这两种菌株均具有多药耐药（MDR）表型。此外，毒力组学分析证实了这两种菌株均存在铁载体基因 ，此外还有耶尔森菌素和/或大肠菌素 V 编码基因，这些基因通常与人类的高毒力有关。此外，这两种分离株均能够杀死 ，并且与对照菌株相比表现出更高的毒力。总之，这是首次在智利报道 复合体成员的毒力和 MDR 表型趋同，这表明在南美洲存在一个值得特别关注和持续监测的临床问题。
