Lin W L
Kresge Eye Institute, Wayne State University School of Medicine, Detroit, MI 48201.
Acta Neuropathol. 1988;76(4):427-31. doi: 10.1007/BF00686981.
The effect of the polycations, protamine sulfate and poly-L-lysine, on the blood-retinal barrier of rat retinal vessels were studied by retrograde perfusion through the aorta or by intracarotid perfusion of the polycation followed by the protein tracer, hemoglobin. Protamine sulfate induced swelling of cytoplasmic organelles and diffuse staining of many endothelial cells by tracer molecules which subsequently entered the subendothelial and perivascular areas. Polylysine caused some diffuse staining but no leakage of tracer through the endothelial cell. Occasionally, tracer was found in the interendothelial junction after protamine perfusion. The results indicate that surface charge is important for maintaining membrane integrity of the endothelial cells and that breakdown of the blood-retinal barrier may be due to the cytotoxic effect of protamine on the endothelial cell.
通过经主动脉逆行灌注或经颈内动脉灌注聚阳离子后再灌注蛋白质示踪剂血红蛋白，研究了聚阳离子硫酸鱼精蛋白和聚-L-赖氨酸对大鼠视网膜血管血视网膜屏障的影响。硫酸鱼精蛋白导致细胞质细胞器肿胀，许多内皮细胞被示踪分子弥漫性染色，随后示踪分子进入内皮下和血管周围区域。聚赖氨酸引起一些弥漫性染色，但示踪剂未透过内皮细胞泄漏。硫酸鱼精蛋白灌注后，偶尔在内皮细胞间连接处发现示踪剂。结果表明，表面电荷对于维持内皮细胞的膜完整性很重要，血视网膜屏障的破坏可能是由于鱼精蛋白对内皮细胞的细胞毒性作用。
