Center for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia
Turk J Med Sci. 2020 Feb 13;50(1):267-270. doi: 10.3906/sag-1909-4.
BACKGROUND/AIM: The aim of this study was to find out characteristics and patterns of the spread of Corynebacterium diphtheriae isolated from Jakarta and the surrounding areas, using the whole genome sequencing (WGS) technique and multilocus sequence typing (MLST) approach.
The study samples consisted of 86 C. diphtheriae isolates, which were isolated from diphtheria patients and close contacts of patients. The DNA sequencing was carried out using the WGS technique. Data conversion applied the U-gene software. Molecular typing was conducted through the MLST approach, then followed by online data analysis.
The results showed that as many as 43 (50%) of all samples examined were new types with the same allele profile, namely 9-1- 13-4-3-3-4. New sequence type C. diphtheriae is registered in the MLST global database as ST534 based on the allele profile. The tox gene analysis in 43 isolates with ST534 indicated that there were three mutation positions, all of which were silent mutations.
The main cause of diphtheria in Jakarta and the surrounding areas is a new sequence type of C. diphtheriae registered as ST534.
背景/目的：本研究旨在通过全基因组测序（WGS）技术和多位点序列分型（MLST）方法，找出从雅加达及周边地区分离出的白喉棒状杆菌的传播特征和模式。
研究样本包括 86 株白喉棒状杆菌分离株，这些分离株来自白喉患者及其密切接触者。采用 WGS 技术进行 DNA 测序。应用 U-基因软件进行数据转换。通过 MLST 方法进行分子分型，然后进行在线数据分析。
结果表明，在所检查的所有样本中，多达 43 种（50%）为新型，具有相同的等位基因谱，即 9-1-13-4-3-3-4。新的白喉棒状杆菌序列型 C. diphtheriae 以等位基因谱为基础，在 MLST 全球数据库中注册为 ST534。对 43 株具有 ST534 的毒素基因分析表明，有三个突变位置，均为沉默突变。
雅加达及周边地区白喉的主要原因是一种新的序列型白喉棒状杆菌，被登记为 ST534。
