Northover A M
Department of Pharmacology, School of Pharmacy, Leicester Polytechnic, UK.
Agents Actions. 1988 Jul;24(3-4):351-5. doi: 10.1007/BF02028293.
Histamine (300 microM), bradykinin (2 microM), prostaglandin E2 (PGE2) (30 microM), or the leukotrienes (LT) C4 and E4 (1 microM) but not D4 (1 microM) applied in vitro have been shown to change the shape of endothelial cells lining the guinea pig isolated thoracic inferior vena cava. All caused the formation of inter-endothelial cell gaps. Pre-treatment with either indomethacin (100 microM) or verapamil (20 microM) reduced the effects of these compounds. It is suggested that indomethacin and verapamil act by reducing the amount of intracellular calcium available for the shortening of contractile protein filaments within endothelial cells.
已证实，体外应用组胺（300微摩尔）、缓激肽（2微摩尔）、前列腺素E2（PGE2）（30微摩尔）或白三烯（LT）C4和E4（1微摩尔）而非D4（1微摩尔），可改变豚鼠离体胸段下腔静脉内皮细胞的形态。所有这些物质都会导致内皮细胞间出现间隙。用吲哚美辛（100微摩尔）或维拉帕米（20微摩尔）预处理可减弱这些化合物的作用。提示吲哚美辛和维拉帕米的作用机制是减少内皮细胞内可用于收缩蛋白丝缩短的细胞内钙含量。
