Department of Physiology, School of Medicine, Kyung Hee University, Seoul, South Korea.
Methods Mol Biol. 2020;2091:131-135. doi: 10.1007/978-1-0716-0167-9_11.
Optical molecular and cellular imaging has provided considerable information in various life science research fields. In particular, pHluorin-based assays allow us to specifically investigate presynaptic physiological processes, such as synaptic transmission and synaptic retrieval. Here, I describe quantitative synaptic transmission in primary cultured hippocampal neurons using a vGlut-pHluorin system combined with a high-fidelity optical imaging system and its regulation by inositol pyrophosphate.
光学分子和细胞成像在各个生命科学研究领域提供了大量信息。特别是，基于 pHluorin 的测定方法使我们能够专门研究突触前生理过程，如突触传递和突触回收。在这里，我描述了使用 vGlut-pHluorin 系统结合高保真光学成像系统以及肌醇六磷酸对其调节的原代培养海马神经元中的定量突触传递。
