College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China; Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of TCM, Guangzhou, China; Engineering & Technology Research Center for Chinese Materia Medica Quality of the Universities of Guangdong Province, Guangzhou 510006, China.
College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Carbohydr Polym. 2016 Jun 25;144:106-14. doi: 10.1016/j.carbpol.2016.02.030. Epub 2016 Feb 11.
To explore the chemicals responsible for the health benefits of guava, water-soluble polysaccharides were extracted including GP90 and P90. They exhibited excellent α-glucosidase inhibition activity with an EC50 of 2.27μg/mL and 0.18mg/mL. This suggests that their activities were 1379- and 17-fold higher than the positive control. The DPPH scavenging activities of GP90 was even higher than Vc at some concentrations. Upon further isolation, a novel polysaccharide termed GB90-1B was obtained. Monosaccharide analysis, methylation analysis, and NMR were used to analyze the structural characterization of GB90-1B. Structural analysis revealed that its backbone consisted of (1→5)-linked-α-l-arabinose, (1→2,3,5)-linked-α-l-arabinose and (1→3)-linked-α-l-arabinose. Branch linkages included (1→6)-linked-α-d-glucose, (1→)-linked-α-d-glucose and (1→)-linked-α-l-arabinose. The structure of the repeating unit of GP90-1B was predicted.
为了探索番石榴有益健康的化学成分，提取了水溶性多糖，包括 GP90 和 P90。它们表现出优异的α-葡萄糖苷酶抑制活性，EC50 分别为 2.27μg/mL 和 0.18mg/mL。这表明它们的活性分别比阳性对照高 1379 倍和 17 倍。在某些浓度下，GP90 的 DPPH 清除活性甚至高于 Vc。进一步分离得到一种新型多糖，命名为 GB90-1B。通过单糖分析、甲基化分析和 NMR 对 GB90-1B 的结构特征进行了分析。结构分析表明，其主链由（1→5）-连接的-α-l-阿拉伯糖、（1→2,3,5）-连接的-α-l-阿拉伯糖和（1→3）-连接的-α-l-阿拉伯糖组成。支链连接包括（1→6）-连接的-α-d-葡萄糖、（1→）-连接的-α-d-葡萄糖和（1→）-连接的-α-l-阿拉伯糖。预测了 GP90-1B 的重复单元结构。
