评估单宁对吸入棉纺厂粉尘所致急性肺部炎症反应的作用。
Evaluation of the contribution of tannin to the acute pulmonary inflammatory response against inhaled cotton mill dust.
作者信息
Lauque D E, Hempel S L, Schroeder M A, Hyatt R E, Rohrbach M S
机构信息
Thoracic Diseases Research Unit, Mayo Clinic/Foundation, Rochester, Minnesota 55905.
出版信息
Am J Pathol. 1988 Oct;133(1):163-72.
Anesthetized, intubated, and mechanically ventilated rabbits were exposed to aerosolized saline, cotton dust extract (CDE), or tannin for 5 minutes and lavaged 4 hours after exposure. Cell numbers and types present in the bronchoalveolar lavage fluid (BALF) were determined and the concentrations of thromboxane A2 (TxA2) and prostaglandin F2-alpha (PGF2-alpha) in the BALF were also analyzed. The saline control animals had increased numbers and percentage of polymorphonuclear leukocytes (PMN) in the BALF as well as increased levels of TxB2 and PGF2-alpha compared with unexposed animals. Exposure to CDE further increased the number and percentage of PMN and the level of PGF2-alpha but had no effect on TxA2 levels when compared with control animals. Tannin exposure increased PGF2-alpha levels to the same extent as CDE exposure. PMN also increased but to a lesser extent than with CDE. These results indicate that the inflammatory response to CDE is only partially due to the tannin present in CDE.
将麻醉、插管并进行机械通气的兔子暴露于雾化盐水、棉尘提取物（CDE）或单宁中5分钟，暴露后4小时进行灌洗。测定支气管肺泡灌洗液（BALF）中的细胞数量和类型，并分析BALF中血栓素A2（TxA2）和前列腺素F2-α（PGF2-α）的浓度。与未暴露的动物相比，盐水对照动物的BALF中多形核白细胞（PMN）数量和百分比增加，TxB2和PGF2-α水平也升高。与对照动物相比，暴露于CDE会进一步增加PMN的数量和百分比以及PGF2-α的水平，但对TxA2水平没有影响。单宁暴露使PGF2-α水平升高的程度与CDE暴露相同。PMN也增加了，但程度小于CDE暴露。这些结果表明，对CDE的炎症反应仅部分归因于CDE中存在的单宁。
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