Filho S A, Pereira de Almeida E R, Gander E S
Acta Trop. 1978 Sep;35(3):229-37.
Cultures of Trypanosoma cruzi have been exposed to the drugs hydroxyurea and colchicine. We found that hydroxyurea (200 microgram/ml) completely inhibited growth and differentiation of T. cruzi Y strain. Colchicine (200 microgram/ml) reduced the growth of T. cruzi 30% and stimulated cell differentiation from epimastigotes to trypomastigotes. Furthermore it caused anuclear cells with apparently intact kinetoplasts. The possible use of these anuclear forms in studies on kinetoplast DNA organization and expression is suggested.
克氏锥虫培养物已暴露于羟基脲和秋水仙碱药物中。我们发现，羟基脲（200微克/毫升）完全抑制了克氏锥虫Y株的生长和分化。秋水仙碱（200微克/毫升）使克氏锥虫的生长减少了30%，并刺激细胞从无鞭毛体分化为上鞭毛体。此外，它还导致了具有明显完整动基体的无核细胞。有人提出这些无核形式可能用于动基体DNA组织和表达的研究。
