克氏锥虫：2(3)-叔丁基-4-羟基茴香醚（BHA）对几种株型的前鞭毛体和锥鞭毛体形式的杀锥虫作用

Trypanosoma cruzi: trypanocidal effect of 2(3)-tert-butyl-4-hydroxyanisole (BHA) on several strains of epimastigote and trypomastigote forms.

作者信息

Aldunate J, Ojeda J M, Repetto Y, Letelier M E, Spencer P

机构信息

Department of Biochemistry, Faculty of Medicine, University of Chile, Santiago.

出版信息

Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1994 Oct;109(2):119-27.

PMID:7881815

Abstract

BHA (2(3)-tert-butyl-4-hydroxyanisole) produced inhibition of both culture growth and oxygen consumption, NAD(P) reduction and cytochrome b oxidation, on intact epimastigotes of Trypanosoma cruzi. BHA inhibited respiration and reduced NAD(P) in intact T. cruzi trypomastigotes. Oxidative phosphorylation of in situ mitochondria of epimastigotes was inhibited by BHA and this effect was liberated by the addition of ascorbate+TMPD. The incorporation of rhodamine-123 to mitochondria of living epimastigotes was diminished by BHA. These results suggest that the basis of the trypanocidal effects of BHA could be due to the blockage of the mitochondrial electron transport chain on the segment previous to cytochrome c. We postulate that the mechanism of action of BHA could be by mimicking coenzyme-Q (ubiquinone).

摘要

丁基羟基茴香醚（2(3)-叔丁基-4-羟基茴香醚）对克氏锥虫的完整上鞭毛体的培养生长、氧气消耗、NAD(P)还原及细胞色素b氧化均有抑制作用。丁基羟基茴香醚抑制完整克氏锥虫锥鞭毛体的呼吸作用并降低NAD(P)水平。丁基羟基茴香醚抑制上鞭毛体原位线粒体的氧化磷酸化作用，且加入抗坏血酸+N,N,N',N'-四甲基对苯二胺可消除该作用。丁基羟基茴香醚减少了活体上鞭毛体线粒体对罗丹明-123的摄取。这些结果表明，丁基羟基茴香醚的杀锥虫作用可能是由于其阻断了细胞色素c之前片段的线粒体电子传递链。我们推测，丁基羟基茴香醚的作用机制可能是通过模拟辅酶Q（泛醌）。