Ahi Evran University Faculty of Medicine, Histology and Embryology, Kirsehir, Turkey.
Kyrenia University Faculty of Medicine, Histology and Embriyology, Kyrenia, Cyprus.
Acta Histochem. 2020 Jan;122(1):151465. doi: 10.1016/j.acthis.2019.151465. Epub 2019 Nov 24.
Asherman's syndrome has become a growing problem with the incidence of cesarean and endometrial surgical procedures. A surgical procedure that can damage to the basal layer of the endometrium is formed as intrauterine adhesion and can cause asherman's syndrome. Mesenchymal stem cells (MSCs) are characterized by some characteristics such as non-immunogenic, angiogenic, antifibrotic, antiapoptotic and antiinflammatory properties, also they support tissue repair by secretion of various factors and chemokines in cellular therapy. Exosomes are active paracrine components with a great potential for repairing damaged tissue. Exosomes include many paracrine factors responsible for regeneration and angiogenesis. In this study, 10 newborn Wistar rats were used to obtain MSCs. A total of 24 adult Wistar rats were also used. The rats were divided into 4 groups: untreated control group; asherman control group; asherman + uterine-derived MSCs group; asherman + uterine-derived MSCs-exosomes group. At the end of the experiment, uterine tissues were evaluated by histochemical and immunohistochemical. As a result of MSCs and exosomes treatments, proliferation and vascularization in uterine tissue was increased. It was also shown to reduce fibrosis with masson's trichrome staining. MMP-2 and MMP-9 expression was enhanced by MSC and exosomal therapy; in addition, TIMP-2 expression was decreased. In our study, it was shown that proliferation and vascularization increased and fibrosis decreased in uterus as a result of MSC and exosome treatments. Our results indicate that the exosomal treatment restored the damage of asherman's syndrome at tissue at a shorter time than the MSCs group.
阿舍曼综合征随着剖宫产和子宫内膜手术的发生率不断增加已成为一个日益严重的问题。一种可损伤子宫内膜基底层的手术会形成宫腔粘连，并可能导致阿舍曼综合征。间充质干细胞（MSCs）具有一些特征，如非免疫原性、血管生成、抗纤维化、抗凋亡和抗炎特性，此外，它们还通过细胞疗法分泌各种因子和趋化因子来支持组织修复。外泌体是具有很大潜力修复受损组织的活性旁分泌成分。外泌体包含许多负责再生和血管生成的旁分泌因子。在这项研究中，使用 10 只新生 Wistar 大鼠获得 MSCs。还使用了总共 24 只成年 Wistar 大鼠。大鼠被分为 4 组：未处理对照组；阿舍曼对照组；阿舍曼+子宫来源 MSCs 组；阿舍曼+子宫来源 MSCs-外泌体组。实验结束时，通过组织化学和免疫组织化学评估子宫组织。结果表明，MSCs 和外泌体治疗可增加子宫组织的增殖和血管生成，并通过马松三色染色显示减少纤维化。MMP-2 和 MMP-9 的表达通过 MSC 和外泌体治疗增强，此外 TIMP-2 的表达降低。在我们的研究中，结果表明，MSCs 和外泌体治疗可增加子宫的增殖和血管生成，并减少纤维化。我们的结果表明，外泌体治疗在较短的时间内恢复了阿舍曼综合征在组织中的损伤，比 MSCs 组更快。
