Division of Cardiovascular Medicine, The Gill Heart and Vascular Institute, Superfund Research Center and Center for Appalachian Research in Environmental Sciences, University of Kentucky College of Medicine, Lexington Veterans Affairs Medical Center, Lexington, KY 40536, USA.
Institute of Environmental Health Sciences and Department of Pharmacology, Wayne State University, Detroit, MI 48202, USA.
J Anal Toxicol. 2020 May 18;44(4):339-347. doi: 10.1093/jat/bkz097.
Per- and poly-fluorinated alkyl substances (PFASs) are a large group of synthetic surfactant chemicals with widespread uses in food packaging and textile manufacturing and as the main constituent of aqueous film-forming firefighting foams. PFASs are highly persistent in the environment, and human exposures are extensive with these chemicals detectable in the blood of almost all adult Americans. PFASs exhibit a range of toxic effects in preclinical models. In humans, PFAS exposure has been associated with lower birth weights, decreased immune responses, cancer and impaired fertility and elevated circulating cholesterol levels. We have developed a sensitive high-throughput method for quantification of representative PFAS in human serum and plasma for biomonitoring and epidemiological studies of human health effects of PFAS exposure. The method combines robust and reproducible 96-well plate format sample preparation with ultra-performance liquid chromatography-tandem mass spectrometry. The method was developed, validated and used for targeted measurements of eight short-/long-chain PFAS analytes in human serum. Targeted analytes were measured in 50 microliters of sample using mass-labeled internal standards. Mean spiked recoveries (n = 10) of target analytes for three tiers quality control (QC-low, QC-medium, QC-high) samples ranged from 70 to 127% with 2-14% relative standard deviation (RSD). The average spiked recoveries (n = 10) of surrogates were 79-115% with 8-12% RSD for QC-low, 90-123% with 7-12% RSD for QC-medium and 82-114% with 9-15% RSD for QC-high. The limit of detection for the target compounds was 0.05-0.04 ng/mL. The method was used to reveal regional differences in PFAS exposures in Kentucky residents receiving care at the University of Kentucky Hospitals.
全氟和多氟烷基物质（PFAS）是一大类广泛用于食品包装和纺织制造的合成表面活性剂化学物质，也是水成膜泡沫灭火剂的主要成分。PFAS 在环境中具有高度持久性，人类接触广泛，几乎所有美国成年人的血液中都能检测到这些化学物质。PFAS 在临床前模型中表现出一系列毒性作用。在人类中，PFAS 暴露与较低的出生体重、免疫反应减弱、癌症以及生育能力受损和循环胆固醇水平升高有关。我们开发了一种灵敏的高通量方法，用于定量分析人血清和血浆中的代表性 PFAS，用于监测 PFAS 暴露对人类健康影响的生物监测和流行病学研究。该方法结合了稳健且可重复的 96 孔板格式样品制备与超高效液相色谱-串联质谱法。该方法经过开发、验证，并用于靶向测量人血清中 8 种短/长链 PFAS 分析物。使用质量标记的内标，在 50 微升样品中测量目标分析物。三个质量控制（QC-低、QC-中、QC-高）样品的目标分析物平均加标回收率（n=10）为 70-127%，相对标准偏差（RSD）为 2-14%。替代物的平均加标回收率（n=10）为 QC-低时为 79-115%，RSD 为 8-12%；QC-中时为 90-123%，RSD 为 7-12%；QC-高时为 82-114%，RSD 为 9-15%。目标化合物的检测限为 0.05-0.04ng/mL。该方法用于揭示肯塔基州居民在肯塔基大学医院接受治疗时的 PFAS 暴露的区域差异。
