Sultana S, Jabeen F, Sultana T, Al-Ghanim K A, Al-Misned F, Mahboob S
Department of Zoology, Government College, University, 38000, Faisalabad, Pakistan.
Department of Zoology, College of Science, King Saud University, Kingdom of Saudi Arabia.
Braz J Biol. 2020 Oct-Dec;80(4):823-828. doi: 10.1590/1519-6984.221849.
This study was conducted to assess water pollution by examining DNA fragmentation in selected fish organs (kidney, liver, gills, and muscle tissue) from Wallago attu, Sperata sarwari, Vulgaris vulgaris, and Labeo rohita collected from a known polluted section of the Chenab River, Pakistan, and from a control site. The fish were caught using a gill net and were assigned to three different weight groups (W1, W2, and W3) to study the degree of variation in DNA fragmentation in relation to body weight. In fish from the polluted site, DNA fragmentation was higher in kidney, liver, gills, and muscles, compared to the control. No significant DNA fragmentation was observed in fish collected from the control site. Highly significant (P < 0.01) relationship between body weight and DNA fragmentation was found in the organs of fish procured at the contaminated site. DNA fragmentation in body organs was found to be affected by the concentrations of lead, copper, nickel, and cadmium in W. attu, S. sarwari, L. rohita, and V. vulgarus harvested from Chenab River. DNA fragmentation in different freshwater fish species is therefore a reliable biomarker of water pollution.
本研究旨在通过检测从巴基斯坦杰纳布河已知污染河段及对照点采集的南亚野鲮、萨氏魾、埃及塘鲺和印度魮的特定鱼类器官（肾脏、肝脏、鳃和肌肉组织）中的DNA片段化情况，来评估水污染程度。使用刺网捕获鱼类，并将其分为三个不同体重组（W1、W2和W3），以研究DNA片段化程度与体重的变化关系。与对照相比，污染点鱼类的肾脏、肝脏、鳃和肌肉中的DNA片段化程度更高。在对照点采集的鱼类中未观察到明显的DNA片段化现象。在污染点采集的鱼类器官中，体重与DNA片段化之间存在高度显著（P < 0.01）的关系。从杰纳布河捕获的南亚野鲮、萨氏魾、印度魮和埃及塘鲺体内器官的DNA片段化受铅、铜、镍和镉浓度的影响。因此，不同淡水鱼类物种中的DNA片段化是水污染的可靠生物标志物。
