Department of Pharmaceutical Sciences, University of Michigan, Ann Arbor, MI 48109, USA; Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.
Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.
J Control Release. 2020 Feb;318:264-269. doi: 10.1016/j.jconrel.2019.11.030. Epub 2019 Nov 25.
Constant therapeutic gas phase nitric oxide (NO) delivery is achieved from S-nitrosothiol (RSNO) type NO donor doped silicone rubber films using feedback-controlled photolysis. For photo-release of the NO gas, the intensity of the LED light source is controlled via a PID (proportional-integral-derivative) controller implemented on a microcontroller. The NO concentration within the emitted gas phase is monitored continuously with a commercial amperometric NO gas sensor. NO release was accurately adjustable up to 10 ppm across a broad range of setpoints with response times of roughly 1 min or less. When NO is generated into an air recipient stream, lower NO yields and a comparable level of toxic nitrogen dioxide (NO) formation is observed. However, NO gas generated into an N recipient gas stream can be blended into pure O with very low NO formation. Following scale-up, this technology could be used for point-of-care gas phase NO generation as an alternative for currently used gas cylinder technology for treatment of health conditions where inhaled NO is beneficial, such as pulmonary hypertension, hypoxemia, and cystic fibrosis.
通过反馈控制光解，从掺有 S-亚硝基硫醇 (RSNO) 型一氧化氮 (NO) 供体的硅橡胶薄膜中实现持续的治疗性气相 NO 输送。为了释放 NO 气体，通过微控制器上实现的 PID（比例-积分-微分）控制器来控制 LED 光源的强度。使用商业安培型 NO 气体传感器连续监测发射气相中的 NO 浓度。NO 释放的可调范围非常广泛，设定值可高达 10ppm，响应时间约为 1 分钟或更短。当 NO 生成到空气接收流中时，会观察到较低的 NO 产率和相当水平的有毒二氧化氮 (NO) 形成。然而，将 NO 气体生成到 N 接收气体流中，可以与非常低的 NO 形成混合到纯 O 中。在扩大规模后，这项技术可用于即时气相 NO 生成，作为目前用于治疗吸入 NO 有益的健康状况（如肺动脉高压、低氧血症和囊性纤维化）的气瓶技术的替代方案。
