Dadoune J P, Mayaux M J, Guihard-Moscato M L
Laboratoire d'Histologie-Embryologie-Cytogénétique, Faculté de Médecine Broussais Hôtel-Dieu/Paris, France.
Andrologia. 1988 May-Jun;20(3):211-7.
Human sperm heads which present disturbances of chromatin condensation are stained by acidic aniline, blue. To determine whether the proportion of unstained heads, i.e. with well condensed chromatin, can be considered as an index of sperm quality, a study was undertaken in 157 men during an infertility evaluation. In addition to the usual sperm characteristics, the percentages of unstained heads and of morphologically normal and abnormal forms were concomitantly evaluated. In a total of 15760 spermatozoa, the percentage of unstained heads was much higher in the population of morphologically normal forms than in that of abnormal forms (79.1% and 49.4% respectively, p less than 10(-9]. Among spermatozoa with structural abnormalities, it was much higher in cells with a single anomaly than in those with associated anomalies (53.9% and 40.6% respectively, p less than 10(-9]. When morphology was taken into account, only vitality was found to vary significantly with the percentage of unstained heads.
呈现染色质凝聚异常的人类精子头部会被酸性苯胺蓝染色。为了确定未染色头部（即染色质凝聚良好的头部）的比例是否可被视为精子质量的一个指标，在对157名男性进行不育评估期间开展了一项研究。除了常规的精子特征外，还同时评估了未染色头部的百分比以及形态正常和异常形态的百分比。在总共15760个精子中，形态正常的精子群体中未染色头部的百分比远高于异常形态的精子群体（分别为79.1%和49.4%，p小于10⁻⁹）。在有结构异常的精子中，单一异常细胞中的未染色头部百分比远高于伴有相关异常的细胞（分别为53.9%和40.6%，p小于10⁻⁹）。当考虑形态时，仅发现活力与未染色头部的百分比有显著差异。
