Wong Alexander, Chuan Sandra S, Patton William C, Jacobson John D, Corselli Johannah, Chan Philip J
Department of Gynecology and Obstetrics, Loma Linda University School of Medicine, Loma Linda, California 92354, USA.
Fertil Steril. 2008 Nov;90(5):1999-2002. doi: 10.1016/j.fertnstert.2007.09.026. Epub 2008 Jan 4.
To compare the aniline blue assay with and without eosin, and to correlate the results with pregnancy outcome after intracytoplasmic sperm injection (ICSI) procedure.
A retrospective study.
University-based fertility center.
PATIENT(S): One hundred thirty infertile patients.
INTERVENTION(S): Left-over washed sperm after each ICSI procedure were fixed on glass slides and stained with aniline blue with and without eosin.
MAIN OUTCOME MEASURE(S): Chromatin condensation, pregnancy, and age.
RESULT(S): Percentage chromatin condensation assessed by aniline blue-eosin was higher compared with standard aniline blue (72.4 +/- 2.4% vs. 64.0 +/- 2.4% [mean +/- SEM]). Chromatin condensation was higher in pregnant (86.6 +/- 0.9%) versus nonpregnant (80.9 +/- 2.1%) women age 35 years or more. In younger women, chromatin condensation was not correlated with pregnancy outcome. There was no correlation between chromatin condensation and ICSI fertilization or male age.
CONCLUSION(S): Adding eosin counterstain to aniline blue improved assessment of chromatin condensation, suggesting that the standard assay underestimated chromatin condensation. The association between chromatin condensation and pregnancy in older but not younger women suggests that oocytes of younger women had the capacity to compensate for the immature sperm shortcomings.
比较使用和不使用伊红的苯胺蓝检测法，并将结果与卵胞浆内单精子注射（ICSI）术后的妊娠结局相关联。
一项回顾性研究。
大学附属医院生育中心。
130例不孕患者。
每次ICSI术后剩余的洗涤精子固定于载玻片上，分别用含伊红和不含伊红的苯胺蓝进行染色。
染色质凝聚、妊娠情况和年龄。
与标准苯胺蓝检测法相比，苯胺蓝 - 伊红检测法评估的染色质凝聚百分比更高（72.4±2.4% 对 64.0±2.4% [均值±标准误]）。35岁及以上孕妇的染色质凝聚程度（86.6±0.9%）高于未孕女性（80.9±2.1%）。在年轻女性中，染色质凝聚与妊娠结局无关。染色质凝聚与ICSI受精或男性年龄之间无相关性。
在苯胺蓝中添加伊红复染可改善对染色质凝聚的评估，提示标准检测法低估了染色质凝聚程度。老年而非年轻女性中染色质凝聚与妊娠之间的关联表明，年轻女性的卵母细胞有能力弥补精子不成熟的缺陷。
